ArticleJournal of virology2000
Establishment and characterization of molecular clones of porcine endogenous retroviruses replicating on human cells.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 110 citations in OpenAlex.
- Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus.International journal of molecular sciences · 2026Article
- Analysis of PERV-C superinfection resistance using HA-tagged viruses.Retrovirology · 2023Article
- Isolation of an Ecotropic Porcine Endogenous Retrovirus PERV-C from a Yucatan SLAJournal of virology · 2023Article
- Detergent-Free Decellularization of Notochordal Cell-Derived Matrix Yields a Regenerative, Injectable, and Swellable Biomaterial.ACS biomaterials science & engineering · 2022Article
- Rare isolation of human-tropic recombinant porcine endogenous retroviruses PERV-A/C from Göttingen minipigs.Virology journal · 2022Article
- High Prevalence of Recombinant Porcine Endogenous Retroviruses (PERV-A/Cs) in Minipigs: A Review on Origin and Presence.Viruses · 2021Review
- Absence of interaction between porcine endogenous retrovirus and porcine cytomegalovirus in pig-to-baboon renal xenotransplantation in vivo.Xenotransplantation · 2018Article
- Multiplatform next-generation sequencing identifies novel RNA molecules and transcript isoforms of the endogenous retrovirus isolated from cultured cells.FEMS microbiology letters · 2018Article
- Porcine Endogenous Retrovirus (PERV) - Molecular Structure and Replication Strategy in the Context of Retroviral Infection Risk of Human Cells.Frontiers in microbiology · 2018Review
- Article
- Identification and promoter analysis of PERV LTR subtypes in NIH-miniature pig.Molecules and cells · 2013Article
- Genetically modified pigs for biomedical research.Journal of inherited metabolic disease · 2012Review
- Infection barriers to successful xenotransplantation focusing on porcine endogenous retroviruses.Clinical microbiology reviews · 2012Review
- Influence of chitosan nanofiber scaffold on porcine endogenous retroviral expression and infectivity in pig hepatocytes.World journal of gastroenterology · 2011Article
- Restriction of porcine endogenous retrovirus by porcine APOBEC3 cytidine deaminases.Journal of virology · 2011Article
- Comparison of the age-related porcine endogenous retrovirus (PERV)expression using duplex RT-PCR.Journal of veterinary science · 2009Article
- Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig.Virus genes · 2009Article
- Identification of two distinct structural regions in a human porcine endogenous retrovirus receptor, HuPAR2, contributing to function for viral entry.Retrovirology · 2009Article
- Porcine endogenous retrovirus integration sites in the human genome: features in common with those of murine leukemia virus.Journal of virology · 2006Article
- Reliable classification and recombination analysis of porcine endogenous retroviruses.Virus genes · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of pig xenografts is being considered to alleviate the shortage of allogeneic organs for transplantation. In addition to the problems overcoming immunological and physiological barriers, the existence of numerous porcine microorganisms poses the risk of initiating a xenozoonosis. Recently, different classes of type C porcine endogenous retoviruses (PERV) which are infectious for human cells in vitro have been partially described. We therefore examined whether completely intact proviruses exist that produce infectious and replication-competent virions. Several proviral PERV sequences were cloned and characterized. One molecular PERV class B clone, PERV-B(43), generated infectious particles after transfection into human 293 cells. A second clone, PERV-B(33), which was highly homologous to PERV-B(43), showed a G-to-A mutation in the first start codon (Met to Ile) of the env gene, preventing this provirus from replicating. However, a genetic recombinant, PERV-B(33)/ATG, carrying a restored env start codon, became infectious and could be serially passaged on 293 cells similar to virus clone PERV-B(43). PERV protein expression was detected 24 to 48 h posttransfection (p. t.) using cross-reacting antiserum, and reverse transcriptase activity was found at 12 to 14 days p.t. The transcriptional start and stop sites as well as the splice donor and splice acceptor sites of PERV mRNA were mapped, yielding a subgenomic env transcript of 3. 1 kb. PERV-B(33) and PERV-B(43) differ in the number of copies of a 39-bp segment in the U3 region of the long terminal repeat. Strategies to identify and to specifically suppress or eliminate those proviruses from the pig genome might help in the production of PERV-free animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.