ArticleJournal of virology2000
Generation of cytomegalovirus-specific human T-lymphocyte clones by using autologous B-lymphoblastoid cells with stable expression of pp65 or IE1 proteins: a tool to study the fine specificity of the antiviral response.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02973464 (The Strategy in the Prevention of Renal Post-transplant Cytomegalovirus Infection Among Chinese Population), which is not on this map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Strategy in the Prevention of Renal Post-transplant Cytomegalovirus Infection Among Chinese Population
Who cites it
20 citing papers in PubMed, 75 citations in OpenAlex.
- Immunosequencing identifies signatures of cytomegalovirus exposure history and HLA-mediated effects on the T cell repertoire.Nature genetics · 2017Article
- Multiplex Identification of Antigen-Specific T Cell Receptors Using a Combination of Immune Assays and Immune Receptor Sequencing.PloS one · 2015Article
- Identification of transcription factor AML-1 binding site upstream of human cytomegalovirus UL111A gene.PloS one · 2015Article
- Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Combining next-generation sequencing and immune assays: a novel method for identification of antigen-specific T cells.PloS one · 2013Article
- Persistent viral infection in humans can drive high frequency low-affinity T-cell expansions.Immunology · 2010Article
- Comparisons of CD8+ T cells specific for human immunodeficiency virus, hepatitis C virus, and cytomegalovirus reveal differences in frequency, immunodominance, phenotype, and interleukin-2 responsiveness.Journal of virology · 2009Article
- Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells.Journal of translational medicine · 2008Article
- Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants.PloS one · 2008Article
- Refinement of strategies for the development of a human cytomegalovirus dense body vaccine.Medical microbiology and immunology · 2008Article
- Cytomegalovirus infection in Gambian infants leads to profound CD8 T-cell differentiation.Journal of virology · 2007Article
- CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects.Journal of translational medicine · 2007Article
- Selection of Epstein-Barr virus specific cytotoxic T lymphocytes can be performed with B lymphoblastoid cell lines created in serum-free media.Clinical and experimental immunology · 2006Article
- Simultaneous assessment of cytotoxic T lymphocyte responses against multiple viral infections by combined usage of optimal epitope matrices, anti- CD3 mAb T-cell expansion and "RecycleSpot"Journal of translational medicine · 2005Article
- Cross-reactivity of HLA-B*1801-restricted T-lymphocyte clones with target cells expressing variants of the human cytomegalovirus 72kDa-IE1 protein.Journal of virology · 2003Article
- Mature CD8(+) T lymphocyte response to viral infection during fetal life.The Journal of clinical investigation · 2003Article
- Ex vivo profiling of CD8+-T-cell responses to human cytomegalovirus reveals broad and multispecific reactivities in healthy virus carriers.Journal of virology · 2003Article
- Use of transgenic HLA A*0201/Kb and HHD II mice to evaluate frequency of cytomegalovirus IE1-derived peptide usage in eliciting human CD8 cytokine response.Journal of virology · 2003Article
- Antigens and immunoevasins: opponents in cytomegalovirus immune surveillance.Nature reviews. Immunology · 2002Review
- Ex vivo stimulation and expansion of both CD4(+) and CD8(+) T cells from peripheral blood mononuclear cells of human cytomegalovirus-seropositive blood donors by using a soluble recombinant chimeric protein, IE1-pp65.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytotoxic T lymphocytes (CTLs) play a central role in the control of persistent human cytomegalovirus (HCMV) infection in healthy virus carriers. Previous analyses of the specificity of HCMV-reactive CD8(+) CTLs drawn from in vitro models in which antigen-presenting cells were autologous fibroblasts infected with laboratory HCMV strains have shown focusing of CTL responses against the major tegument protein, pp65. By contrast, the 72-kDa major immediate-early protein (IE1) was identified as a minor target for this response. Here we have studied the fine specificity and T-cell-receptor features of T-cell clones generated against autologous B lymphoblastoid cell lines stably transfected with HCMV cDNA coding for either pp65 or a natural variant of IE1. This strategy allowed efficient generation of T-cell clones against IE1 and pp65 and led to the identification of several new IE1 and pp65 epitopes, including some located in polymorphic regions of IE1. Such an approach may provide relevant information about the characteristics of the CTL response to IE1 and the effect of viral polymorphism on the immune response against HCMV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.