Evidence map›Paper›PMID 10756003›Full record

ReviewClinical microbiology reviews2000

The ins and outs of DNA fingerprinting the infectious fungi.

D R Soll

Open access · greenAbstract readReview
In one paragraph

Review in Clinical microbiology reviews, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
101citing papers in PubMed, 1 pooled it
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

101 citing papers in PubMed, 1 synthesis or guideline pooled it, 332 citations in OpenAlex.

  1. The use of genetic markers in the molecular epidemiology of histoplasmosis: a systematic review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016
    Pooled it
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  17. The Candida pathogenic species complex.Cold Spring Harbor perspectives in medicine · 2014
    Review
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  20. Review

41 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

D R SollDepartment of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA. david-soll@uiowa.edu
University of Iowa · US

Funding

SWITCHING AND CANDIDA PATHOGENESIS--A MOLECULAR ANALYSISR01AI039735 · NIAID · UNIVERSITY OF IOWA · PI SOLL, DAVID R. · 1996 to 2005
$1.5M
ROLE OF ANTIOXIDANT ENZYMES IN ORAL CANCERP50DE010758 · NIDCR · UNIVERSITY OF IOWA · PI SWARTZENDRUBER, DONALD C · 1993 to 1998
–
NIAID NIH HHS AI39735NIDCR NIH HHS DE10758
6 · The paper itself

Abstract

DNA fingerprinting methods have evolved as major tools in fungal epidemiology. However, no single method has emerged as the method of choice, and some methods perform better than others at different levels of resolution. In this review, requirements for an effective DNA fingerprinting method are proposed and procedures are described for testing the efficacy of a method. In light of the proposed requirements, the most common methods now being used to DNA fingerprint the infectious fungi are described and assessed. These methods include restriction fragment length polymorphisms (RFLP), RFLP with hybridization probes, randomly amplified polymorphic DNA and other PCR-based methods, electrophoretic karyotyping, and sequencing-based methods. Procedures for computing similarity coefficients, generating phylogenetic trees, and testing the stability of clusters are then described. To facilitate the analysis of DNA fingerprinting data, computer-assisted methods are described. Finally, the problems inherent in the collection of test and control isolates are considered, and DNA fingerprinting studies of strain maintenance during persistent or recurrent infections, microevolution in infecting strains, and the origin of nosocomial infections are assessed in light of the preceding discussion of the ins and outs of DNA fingerprinting. The intent of this review is to generate an awareness of the need to verify the efficacy of each DNA fingerprinting method for the level of genetic relatedness necessary to answer the epidemiological question posed, to use quantitative methods to analyze DNA fingerprint data, to use computer-assisted DNA fingerprint analysis systems to analyze data, and to file data in a form that can be used in the future for retrospective and comparative studies.

Indexed as

DNA FingerprintingDNA, FungalDNA ProbesFungiHumansMycological Typing TechniquesMycosesPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueSoftwareDNA, FungalDNA Probes

Identifiers

PMID10756003
PMCPMC100156
OpenAlexW2106936504

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.