ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Heteromeric assembly of GABA(B)R1 and GABA(B)R2 receptor subunits inhibits Ca(2+) current in sympathetic neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 105 citations in OpenAlex.
- The role of γ-aminobutyric acid and its receptor in metabolic reprogramming and tumor progression.Biochemistry and biophysics reports · 2026Review
- Crosstalk Between GABAergic Neurotransmission and Inflammatory Cascades in the Post-ischemic Brain: Relevance for Stroke Recovery.Frontiers in cellular neuroscience · 2022Review
- GABABMC cancer · 2018Article
- Homeostatic regulation through GABA and acetylcholine muscarinic receptors of motor trigeminal neurons following sleep deprivation.Brain structure & function · 2017Article
- GABAergic signaling in the rat pineal gland.Journal of pineal research · 2016Article
- Role of principal ionotropic and metabotropic receptors in visceral pain.Journal of neurogastroenterology and motility · 2015Article
- Glutamine synthetase stability and subcellular distribution in astrocytes are regulated by γ-aminobutyric type B receptors.The Journal of biological chemistry · 2014Article
- Concomitant facilitation of GABAA receptors and KV7 channels by the non-opioid analgesic flupirtine.British journal of pharmacology · 2012Article
- Drug management of visceral pain: concepts from basic research.Pain research and treatment · 2012Article
- The level and distribution of the GABA(B)R1 and GABA(B)R2 receptor subunits in the rat's inferior colliculus.Frontiers in neural circuits · 2012Article
- Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons.The Journal of biological chemistry · 2009Article
- MYCN-non-amplified metastatic neuroblastoma with good prognosis and spontaneous regression: a molecular portrait of stage 4S.Molecular oncology · 2008Article
- Spinal nerve ligation does not alter the expression or function of GABA(B) receptors in spinal cord and dorsal root ganglia of the rat.Neuroscience · 2006Article
- Compartmental localization of gamma-aminobutyric acid type B receptors in the cholinergic circuitry of the rabbit retina.The Journal of comparative neurology · 2005Article
- Redistribution of GABAB(1) protein and atypical GABAB responses in GABAB(2)-deficient mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- G-protein-coupled receptors for neurotransmitter amino acids: C-terminal tails, crowded signalosomes.The Biochemical journal · 2002Review
- A single subunit (GB2) is required for G-protein activation by the heterodimeric GABA(B) receptor.The Journal of biological chemistry · 2002Article
- GABA(B2) is essential for g-protein coupling of the GABA(B) receptor heterodimer.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- C-terminal interaction is essential for surface trafficking but not for heteromeric assembly of GABA(b) receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- The C-terminal domains of the GABA(b) receptor subunits mediate intracellular trafficking but are not required for receptor signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Neuronal GABA(B) receptors regulate calcium and potassium currents via G-protein-coupled mechanisms and play a critical role in long-term inhibition of synaptic transmission in the CNS. Recent studies have demonstrated that assembly of GABA(B) receptor GABA(B)R1 and GABA(B)R2 subunits into functional heterodimers is required for coupling to potassium channels in heterologous systems. However whether heterodimerization is required for the coupling of GABA(B) receptors to effector systems in neurons remains to be established. To address this issue, we have studied the coupling of recombinant GABA(B) receptors to endogenous Ca(2+) channels in superior cervical ganglion (SCG) neurons using nuclear microinjection to introduce both sense and antisense expression constructs. Patch-clamp recording from neurons injected with both GABA(B)R1a/1b and GABA(B)R2 cDNAs or with GABA(B)R2 alone produced marked baclofen-mediated inhibition of Ca(2+) channel currents via a pertussis toxin-sensitive mechanism. The actions of baclofen were blocked by CGP62349, a specific GABA(B) antagonist, and were voltage dependent. Interestingly, SCGs were found to express abundantly GABA(B)R1 but not GABA(B)R2 at the protein level. To determine whether heterodimerization of GABA(B)R1 and GABA(B)R2 subunits was required for Ca(2+) inhibition, the GABA(B)R2 expression construct was microinjected with a GABA(B)R1 antisense construct. This resulted in a dramatic decrease in the levels of the endogenous GABA(B)R1 protein and a marked reduction in the inhibitory effects of baclofen on Ca(2+) currents. Therefore our results suggest that in neurons heteromeric assemblies of GABA(B)R1 and GABA(B)R2 are essential to mediate GABAergic inhibition of Ca(2+) channel currents.
Indexed as
Identifiers
10751439PMC6772195W1536840470What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.