ArticleMolecular biology of the cell2000
Time-lapse video microscopy analysis reveals astral microtubule detachment in the yeast spindle pole mutant cnm67.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- Cell trivision of hyperploid cells.DNA and cell biology · 2013Article
- Organization of organelles within hyphae of Ashbya gossypii revealed by electron tomography.Eukaryotic cell · 2013Article
- Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body.Molecular biology of the cell · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
- MHO1, an evolutionarily conserved gene, is synthetic lethal with PLC1; Mho1p has a role in invasive growth.PloS one · 2012Article
- Independent modulation of the kinase and polo-box activities of Cdc5 protein unravels unique roles in the maintenance of genome stability.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4.PloS one · 2011Article
- Structural mutants of the spindle pole body cause distinct alteration of cytoplasmic microtubules and nuclear dynamics in multinucleated hyphae.Molecular biology of the cell · 2010Article
- Neurodegeneration mutations in dynactin impair dynein-dependent nuclear migration.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast.Molecular and cellular biology · 2008Article
- A Bir1p Sli15p kinetochore passenger complex regulates septin organization during anaphase.Molecular biology of the cell · 2007Article
- The anaphase-promoting complex/cyclosome is required for anaphase progression in multinucleated Ashbya gossypii cells.Eukaryotic cell · 2007Article
- The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.Molecular biology of the cell · 2007Article
- AgSwe1p regulates mitosis in response to morphogenesis and nutrients in multinucleated Ashbya gossypii cells.Molecular biology of the cell · 2006Article
- Asynchronous nuclear division cycles in multinucleated cells.The Journal of cell biology · 2006Article
- A novel role for the CBF3 kinetochore-scaffold complex in regulating septin dynamics and cytokinesis.The Journal of cell biology · 2005Article
- Deletion of the dynein heavy-chain gene DYN1 leads to aberrant nuclear positioning and defective hyphal development in Candida albicans.Eukaryotic cell · 2004Article
- A Ras-like GTPase is involved in hyphal growth guidance in the filamentous fungus Ashbya gossypii.Molecular biology of the cell · 2004Article
- Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharomyces cerevisiae cnm67Delta cells lack the spindle pole body (SPB) outer plaque, the main attachment site for astral (cytoplasmic) microtubules, leading to frequent nuclear segregation failure. We monitored dynamics of green fluorescent protein-labeled nuclei and microtubules over several cell cycles. Early nuclear migration steps such as nuclear positioning and spindle orientation were slightly affected, but late phases such as rapid oscillations and insertion of the anaphase nucleus into the bud neck were mostly absent. Analyzes of microtubule dynamics revealed normal behavior of the nuclear spindle but frequent detachment of astral microtubules after SPB separation. Concomitantly, Spc72 protein, the cytoplasmic anchor for the gamma-tubulin complex, was partially lost from the SPB region with dynamics similar to those observed for microtubules. We postulate that in cnm67Delta cells Spc72-gamma-tubulin complex-capped astral microtubules are released from the half-bridge upon SPB separation but fail to be anchored to the cytoplasmic side of the SPB because of the absence of an outer plaque. However, successful nuclear segregation in cnm67Delta cells can still be achieved by elongation forces of spindles that were correctly oriented before astral microtubule detachment by action of Kip3/Kar3 motors. Interestingly, the first nuclear segregation in newborn diploid cells never fails, even though astral microtubule detachment occurs.
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