ArticleMolecular biology of the cell2000
A novel helicase-type protein in the nucleolus: protein NOH61.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- Annotation and functional prediction of RNA helicases inIMA fungus · 2025Review
- RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.Cell death and differentiation · 2022Article
- DDX56 inhibits PRV replication through regulation of IFN-β signaling pathway by targeting cGAS.Frontiers in microbiology · 2022Article
- DEAD-ly Affairs: The Roles of DEAD-Box Proteins on HIV-1 Viral RNA Metabolism.Frontiers in cell and developmental biology · 2022Review
- DDX56 modulates post-transcriptional Wnt signaling through miRNAs and is associated with early recurrence in squamous cell lung carcinoma.Molecular cancer · 2021Article
- Human DDX56 protein interacts with influenza A virus NS1 protein and stimulates the virus replication.Genetics and molecular biology · 2021Article
- Tissue-Resident-Memory CD8Frontiers in immunology · 2021Article
- Article
- Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex.Stem cell research & therapy · 2020Article
- Role of RNA-binding proteins during the late stages of Flavivirus replication cycle.Virology journal · 2020Review
- Review
- DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.Journal of cell science · 2019Article
- Nonsense-mediated mRNA decay factor Upf2 exists in both the nucleoplasm and the cytoplasm.Molecular medicine reports · 2016Article
- Identification and in silico analysis of cattle DExH/D box RNA helicases.SpringerPlus · 2016Article
- Identification of AMP-activated protein kinase targets by a consensus sequence search of the proteome.BMC systems biology · 2015Article
- DExD/H-box RNA helicases in ribosome biogenesis.RNA biology · 2013Review
- The helicase activity of DDX56 is required for its role in assembly of infectious West Nile virus particles.Virology · 2012Article
- Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles.Molecular & cellular proteomics : MCP · 2011Article
- The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus.Journal of virology · 2011Article
- Dead-box proteins: a family affair--active and passive players in RNP-remodeling.Nucleic acids research · 2006Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the identification, cDNA cloning, and molecular characterization of a novel, constitutive nucleolar protein. The cDNA-deduced amino acid sequence of the human protein defines a polypeptide of a calculated mass of 61.5 kDa and an isoelectric point of 9.9. Inspection of the primary sequence disclosed that the protein is a member of the family of "DEAD-box" proteins, representing a subgroup of putative ATP-dependent RNA helicases. ATPase activity of the recombinant protein is evident and stimulated by a variety of polynucleotides tested. Immunolocalization studies revealed that protein NOH61 (nucleolar helicase of 61 kDa) is highly conserved during evolution and shows a strong accumulation in nucleoli. Biochemical experiments have shown that protein NOH61 synthesized in vitro sediments with approximately 11.5 S, i.e., apparently as homo-oligomeric structures. By contrast, sucrose gradient centrifugation analysis of cellular extracts obtained with buffers of elevated ionic strength (600 mM NaCl) revealed that the solubilized native protein sediments with approximately 4 S, suggestive of the monomeric form. Interestingly, protein NOH61 has also been identified as a specific constituent of free nucleoplasmic 65S preribosomal particles but is absent from cytoplasmic ribosomes. Treatment of cultured cells with 1) the transcription inhibitor actinomycin D and 2) RNase A results in a complete dissociation of NOH61 from nucleolar structures. The specific intracellular localization and its striking sequence homology to other known RNA helicases lead to the hypothesis that protein NOH61 might be involved in ribosome synthesis, most likely during the assembly process of the large (60S) ribosomal subunit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.