ArticleThe Biochemical journal2000
Expression and characterization of a recombinant cysteine proteinase of Leishmania mexicana.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 72 citations in OpenAlex.
- Computational Investigation of the Potential Antileishmanial Mechanism of the Nitroindazole Derivative VATR131.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Advances in Cysteine Protease B Inhibitors for Leishmaniasis Treatment.Current drug targets · 2025Review
- Novel Selective and Low-Toxic Inhibitor ofBiomolecules · 2022Article
- Design, synthesis and biological evaluation of N-oxide derivatives with potent in vivo antileishmanial activity.PloS one · 2021Article
- Identification and characteristics of a cathepsin L-like cysteine protease from Clonorchis sinensis.Parasitology research · 2019Article
- Development of a New Antileishmanial Aziridine-2,3-Dicarboxylate-Based Inhibitor with High Selectivity for Parasite Cysteine Proteases.Antimicrobial agents and chemotherapy · 2016Article
- Synthesis of C3/C1-Substituted Tetrahydroisoquinolines.Molecules (Basel, Switzerland) · 2015Article
- Identification and characterization of a cathepsin-L-like peptidase in Eimeria tenella.Parasitology research · 2014Article
- Heparin modulates the endopeptidase activity of Leishmania mexicana cysteine protease cathepsin L-Like rCPB2.8.PloS one · 2013Article
- Identification of semicarbazones, thiosemicarbazones and triazine nitriles as inhibitors of Leishmania mexicana cysteine protease CPB.PloS one · 2013Article
- Identification of lead compounds targeting the cathepsin B-like enzyme of Eimeria tenella.Antimicrobial agents and chemotherapy · 2012Article
- Article
- Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model.Eukaryotic cell · 2007Article
- The structure of Leishmania mexicana ICP provides evidence for convergent evolution of cysteine peptidase inhibitors.The Journal of biological chemistry · 2006Article
- Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli.The Biochemical journal · 2005Article
- Regulated degradation of an endoplasmic reticulum membrane protein in a tubular lysosome in Leishmania mexicana.Molecular biology of the cell · 2001Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major cysteine proteinase (CPB) of Leishmania mexicana, that is predominantly expressed in the form of the parasite that causes disease in mammals, has been overexpressed in Escherichia coli and purified from inclusion bodies to apparent homogeneity. The CPB enzyme, CPB2.8, was expressed as an inactive pro-form lacking the characteristic C-terminal extension (CPB2.8DeltaCTE). Pro-region processing was initiated during protein refolding and proceeded through several intermediate stages. Maximum enzyme activity accompanied removal of the entire pro-region. This was facilitated by acidification. Purified mature enzyme gave a single band on SDS/PAGE and gelatin SDS/PAGE gels, co-migrated with native enzyme in L. mexicana lysates, and had the same N-terminal sequence as the native enzyme. The procedure yielded >3.5 mg of active enzyme per litre of E. coli culture.
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