ArticleMolecular pathology : MP1999
PCR based detection of mycobacteria in paraffin wax embedded material routinely processed for morphological examination.
Article in Molecular pathology : MP, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 33 citations in OpenAlex.
- XpertAfrican journal of laboratory medicine · 2019Article
- Cutaneous Tuberculosis: Clinicopathologic Arrays and Diagnostic Challenges.Dermatology research and practice · 2018Review
- Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis?Journal of pathology and translational medicine · 2016Article
- Rapid, sensitive, and specific detection of Mycobacterium tuberculosis complex by real-time PCR on paraffin-embedded human tissues.The Journal of molecular diagnostics : JMD · 2011Article
- Sarcoid-like lesions associated with the immune restoration inflammatory syndrome in AIDS: absence of polymerase chain reaction detection of Mycobacterium tuberculosis in granulomas isolated by laser capture microdissection.Virchows Archiv : an international journal of pathology · 2006Article
- Mycobacterial Infections of the Head and Neck.Current infectious disease reports · 2001Article
- A case of tuberculosis verrucosa cutis in Brazil undiagnosed for 15 years.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe incidence of mycobacterial infections has increased during the past five years. A prompt diagnosis is indispensable for initiating appropriate treatment. Because culturing of mycobacteria takes three to six weeks and sensitivity of microscopic detection of acid fast bacilli is low, amplification methods provide promising possibilities. Recently, the polymerase chain reaction (PCR) has been shown to be useful for confirming a mycobacterial infection, especially in cases with unexpected histological findings or lack of suitable material for culturing.
aimsTo evaluate the impact of PCR based techniques in the detection of mycobacterial infections in uncultured routine histological specimens as an alternative to surgical pathology.
methodsTwo hundred and twenty nine formalin fixed and paraffin wax embedded samples from 141 patients with clinical or histological suspicion of a mycobacterial infection were investigated using three different PCR assays and Southern blotting. PCR results were compared with histology and culture and the patients' clinical findings.
resultsWhen using culture as the reference method, the sensitivity for the detection of mycobacteria of the tuberculosis complex was 90%, specificity was 92%, the positive predictive value was 81%, and the negative predictive value was 96%. The sensitivity for the detection of nontuberculous mycobacteria was 100% and specificity was 78%, the positive predictive value was 26%, and the negative predictive value was 100%. The patients' clinical findings supported the PCR positive results, indicating a mycobacterial infection in 11 of 18 initially culture negative cases and in 21 of 35 PCR positive cases without culture results.
conclusionsThese results indicate that PCR based techniques are sensitive, specific, and rapid methods for the detection of mycobacteria in routinely processed paraffin wax embedded and formalin fixed histological samples.
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