ArticleThe Journal of physiology2000
ATP stimulates Cl- secretion and reduces amiloride-sensitive Na+ absorption in M-1 mouse cortical collecting duct cells.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 113 citations in OpenAlex.
- Abnormal purinergic signaling contributes to development of renal cysts in autosomal dominant polycystic kidney disease.Purinergic signalling · 2026Article
- Renal Ghrelin-Family GPR39 Receptor and Urinary Concentrating Ability.Journal of the American Society of Nephrology : JASN · 2025Article
- In Search of a Sensor: How Does COAmerican journal of respiratory cell and molecular biology · 2021Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- ATP release into ADPKD cysts via pannexin-1/P2X7 channels decreases ENaC activity.Biochemical and biophysical research communications · 2019Article
- Mechanism of luminal ATP activated chloride secretion in a polarized epithelium.The journal of physiological sciences : JPS · 2019Article
- Loss of barrier integrity in alveolar epithelial cells downregulates ENaC expression and activity via CaPflugers Archiv : European journal of physiology · 2018Article
- Functional and therapeutic importance of purinergic signaling in polycystic kidney disease.American journal of physiology. Renal physiology · 2016Review
- New Insights into the Molecular Mechanisms Targeting Tubular Channels/Transporters in PKD Development.Kidney diseases (Basel, Switzerland) · 2016Review
- Chloride secretion by renal collecting ducts.Current opinion in nephrology and hypertension · 2015Article
- The dark side of extracellular ATP in kidney diseases.Journal of the American Society of Nephrology : JASN · 2015Review
- Regulation of renal function and blood pressure control by P2 purinoceptors in the kidney.Current opinion in pharmacology · 2015Review
- Basolateral P2X₄channels stimulate ENaC activity in Xenopus cortical collecting duct A6 cells.American journal of physiology. Renal physiology · 2014Article
- CFTR and TNR-CFTR expression and function in the kidney.Biophysical reviews · 2014Review
- Chronic ethanol exposure alters the lung proteome and leads to mitochondrial dysfunction in alveolar type 2 cells.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- Differential effects of extracellular ATP on chloride transport in cortical collecting duct cells.American journal of physiology. Renal physiology · 2012Article
- P2Y receptors and kidney function.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Activation of P2Y1 and P2Y2 receptors induces chloride secretion via calcium-activated chloride channels in kidney inner medullary collecting duct cells.American journal of physiology. Renal physiology · 2011Article
- Regulation of renal NaCl and water transport by the ATP/UTP/P2Y2 receptor system.American journal of physiology. Renal physiology · 2011Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
1. Using equivalent short circuit current (ISC) measurements we examined the effect of extracellular ATP on transepithelial ion transport in M-1 mouse cortical collecting duct cells. Apical addition of ATP produced a rapid transient peak increase in ISC. This was followed by a fall below basal ISC due to a reduction in the amiloride-sensitive ISC component. 2. The ATP-induced ISC increase was preserved in the presence of apical amiloride while it was reduced in the absence of extracellular Cl- and in the presence of the apical Cl- channel blockers diphenylamine-2-carboxylic acid (DPC, 1 mM), DIDS (300 microM) and niflumic acid (100 microM). 3. The stimulatory effect of apical ATP on ISC was concentration dependent with an EC50 of about 0.6 microM. Basolateral ATP elicited a similar ISC response. Experiments using the ATP scavenger hexokinase demonstrated that the ATP effects were elicited via separate apical and basolateral receptors. 4. ATP and UTP applied to either the apical or the basolateral bath equi-potently stimulated ISC while 'purified' ADP and UDP had no effect consistent with P2Y2 purinoceptors, the expression of which was confirmed using RT-PCR. 5. Intracellular calcium concentration ([Ca2+]i) measurements using fura-2 demonstrated that ATP and UTP elicited a rise in [Ca2+]i with EC50 values of 1.1 and 0.6 microM, respectively. The shape and time course of the calcium response were similar to those of the ISC response. The peak ISC response was preserved in the nominal absence of extracellular calcium but was significantly reduced in cells pre-incubated with the calcium chelator BAPTA AM. 6. We conclude that in M-1 cells extracellular ATP reduces amiloride-sensitive Na+ absorption and stimulates Cl- secretion via calcium-activated Cl- channels through activation of P2Y2 purinoreceptors located in the apical and basolateral membrane.
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