ArticleThe Journal of physiology2000
Multiple pathways responsible for the stretch-induced increase in Ca2+ concentration in toad stomach smooth muscle cells.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Stretch-Induced Increase in CaJournal of the American Heart Association · 2025Article
- Article
- Mechanotransduction in gastrointestinal smooth muscle cells: role of mechanosensitive ion channels.American journal of physiology. Gastrointestinal and liver physiology · 2021Review
- Role of BKBioMed research international · 2016Article
- Article
- Local membrane deformation and micro-injury lead to qualitatively different responses in osteoblasts.F1000Research · 2014Article
- Resting tension affects eNOS activity in a calcium-dependent way in airways.Mediators of inflammation · 2007Article
- Estimating the sensitivity of mechanosensitive ion channels to membrane strain and tension.Biophysical journal · 2004Article
- Induced automaticity in isolated rat atrial cells by incorporation of a stretch-activated conductance.Pflugers Archiv : European journal of physiology · 2004Article
- Role of sarcoplasmic reticulum in control of membrane potential and nitrergic response in opossum lower esophageal sphincter.British journal of pharmacology · 2003Article
- Hyposmotic membrane stretch potentiated muscarinic receptor agonist-induced depolarization of membrane potential in guinea-pig gastric myocytes.World journal of gastroenterology · 2002Article
- Stretch-induced calcium release in smooth muscle.The Journal of general physiology · 2002Article
- Single cell mechanotransduction and its modulation analyzed by atomic force microscope indentation.Biophysical journal · 2002Article
- Visualization of Ca2+ entry through single stretch-activated cation channels.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
1. A digital imaging microscope with fura-2 as the Ca2+ indicator was used to determine the sources for the rise in intracellular calcium concentration ([Ca2+]i) that occurs when the membrane in a cell-attached patch is stretched. Unitary ionic currents from stretch-activated channels and [Ca2+]i images were recorded simultaneously. 2. When suction was applied to the patch pipette to stretch a patch of membrane, Ca2+-permeable cation channels (stretch-activated channels) opened and a global increase in [Ca2+]i occurred, as well as a greater focal increase in the vicinity of the patch pipette. The global changes in [Ca2+]i occurred only when stretch-activated currents were sufficient to cause membrane depolarization, as indicated by the reduction in amplitude of the unitary currents. 3. When Ca2+ was present only in the pipette solution, just the focal change in [Ca2+]i was obtained. This focal change was not seen when the contribution from Ca2+ stores was eliminated using caffeine and ryanodine. 4. These results suggest that the opening of stretch-activated channels allows ions, including Ca2+, to enter the cell. The entry of positive charge triggers the influx of Ca2+ into the cell by causing membrane depolarization, which presumably activates voltage-gated Ca2+ channels. The entry of Ca2+ through stretch-activated channels is also amplified by Ca2+ release from internal stores. This amplification appears to be greater than that obtained by activation of whole-cell Ca2+ currents. These multiple pathways whereby membrane stretch causes a rise in [Ca2+]i may play a role in stretch-induced contraction, which is a characteristic of many smooth muscle tissues.
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Registered trials
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