ArticleJournal of clinical microbiology2000
Evaluation of phenotypic markers for selection and identification of Candida dubliniensis.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00692783 (Incidence of Oral Candidiasis, Prevalence of Candida Dubliniensis in HIV Patients and In-vitro Azole Susceptibility.), which is not on this map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Incidence of Oral Candidiasis, Prevalence of Candida Dubliniensis in HIV Patients and In-vitro Azole Susceptibility. (I.C.O.N.I.C.)
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- [Species differentiation of yeasts of the genus Malassezia with Fourier transform infrared spectroscopy].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2006Trial
- Exploring the Biology, Virulence, and General Aspects ofInfection and drug resistance · 2024Review
- A novel rRNA hybridization-based approach to rapid, accurate Candida identification directly from blood culture.Medical mycology · 2022Article
- Identification of Fungi Isolated from Oral Cavity of Patients with HIV Using MALDI-TOF MS.Journal of clinical medicine · 2021Article
- Overall Prevalence and Prevalence Compared among Psoriasis Treatments of Onychomycosis in Patients with Nail Psoriasis and Fungal Involvement.BioMed research international · 2021Article
- Influence of the Sample Preparation Method in DiscriminatingMolecules (Basel, Switzerland) · 2020Article
- Review
- Comparison of duplex PCR and phenotypic analysis in differentiating Candida dubliniensis from Candida albicans from oral samples.AMB Express · 2017Article
- Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Article
- Issues in identifying germ tube positive yeasts by conventional methods.Journal of clinical laboratory analysis · 2014Article
- Epidemiology, antifungal susceptibility, and pathogenicity of Candida africana isolates from the United Kingdom.Journal of clinical microbiology · 2013Article
- A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Candida dubliniensis: epidemiology and phenotypic methods for identification.Mycopathologia · 2010Review
- Identification of Candida spp. by phenotypic tests and PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Genetic relatedness of subgingival and buccal Candida dubliniensis isolates in immunocompetent subjects assessed by RAPD-PCR.Journal of oral microbiology · 2009Article
- Development and evaluation of a rapid latex agglutination test using a monoclonal antibody to identify Candida dubliniensis colonies.Journal of clinical microbiology · 2006Article
- Supplementation of CHROMagar Candida medium with Pal's medium for rapid identification of Candida dubliniensis.Journal of clinical microbiology · 2005Article
- Detection of Candida dubliniensis in Venezuela.Mycopathologia · 2005Article
- Use of a commercial reagent leads to reduced germ tube production by Candida dubliniensis.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficiency virus (HIV)-infected patients were cultured on CHROMagar Candida. Dark green colonies described as being indicative of C. dubliniensis and other green colonies, 170 in total, were isolated. Chlamydospore formation, intracellular beta-D-glucosidase activity, ability to grow at 42 degrees C, carbohydrate assimilation pattern obtained by the API ID 32C, and Fourier transform infrared (FT-IR) spectroscopy were used for phenotypic characterization. Sequencing of the 5' end of the nuclear large-subunit (26S) ribosomal DNA gene was used for definitive species identification for C. dubliniensis. C. dubliniensis was found in 34% of yeast-colonized HIV-infected patients. The color of the colonies on CHROMagar Candida proved to be insufficient for selecting C. dubliniensis, since only 30 of 53 proven C. dubliniensis isolates showed a dark green color in primary cultures. The described typical chlamydospore formation can give only some indication of C. dubliniensis. The assimilation pattern proved to be insufficient to discriminate C. dubliniensis from C. albicans. All C. dubliniensis strains showed no or highly restricted growth at 42 degrees C and a lack of beta-D-glucosidase activity. Unfortunately, atypical C. albicans strains can also exhibit these phenotypic traits. FT-IR spectroscopy combined with hierarchical clustering proved to be as reliable as genotyping for discriminating the two species.
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