ArticleJournal of clinical microbiology2000
Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 40 citations in OpenAlex.
- Drivers of tick community structure in a rhinoceros meta-population in Kenya.International journal for parasitology. Parasites and wildlife · 2026Article
- Molecular detection of Ehrlichia ruminantium and Coxiella burnetii among apparently healthy sheep and goats in eastern Tanzania.Tropical animal health and production · 2025Article
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Ehrlichia ruminantium infects Rhipicephalus microplus in West Africa.Parasites & vectors · 2016Article
- Prevalence of Ehrlichia ruminantium in adult Amblyomma variegatum collected from cattle in Cameroon.Experimental & applied acarology · 2013Article
- Development of loop-mediated isothermal amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium.BMC microbiology · 2010Article
- Geographic distribution and genetic diversity of the Ehrlichia sp. from Panola Mountain in Amblyomma americanum.BMC infectious diseases · 2008Article
- Molecular detection of Ehrlichia ruminantium infection in Amblyomma variegatum ticks in The Gambia.Experimental & applied acarology · 2007Article
- Ehrlichia ruminantium, Sudan.Emerging infectious diseases · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR assay and applied it to field ticks. The assay detected DNA of 37 isolates of C. ruminantium originating from sites throughout the distribution of heartwater and had a specificity of 98% when infected ticks were processed concurrently with uninfected ticks. The assay did not detect DNA of Ehrlichia chaffeensis, which is closely related to C. ruminantium. PCR sensitivity varied with tick infection intensity and was high (97 to 88%) with ticks bearing 10(7) to 10(4) organisms but dropped to 61 and 28%, respectively, with ticks bearing 10(3) and 10(2) organisms. The assay also detected C. ruminantium in collections of Amblyomma hebraeum and Amblyomma variegatum field ticks from 17 heartwater-endemic sites in four southern African countries. Attempts at tick transmission of infection to small ruminants failed with four of these collections. The pCS20 PCR assay is presently the most characterized and reliable test for C. ruminantium in ticks and thus is highly useful for field and laboratory epidemiological investigations of heartwater.
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