ArticleJournal of clinical microbiology2000
A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 67 citations in OpenAlex.
- Promising clinical efficacy of streptomycin-rifampin combination for treatment of buruli ulcer (Mycobacterium ulcerans disease).Antimicrobial agents and chemotherapy · 2007Trial
- A low-cost and versatile paramagnetic bead DNA extraction method forApplied and environmental microbiology · 2024Article
- What about Current Diversity of Mycolactone-Producing Mycobacteria? Implication for the Diagnosis and Treatment of Buruli Ulcer.International journal of molecular sciences · 2023Review
- Molecular detection of Mycobacterium ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of maritime region in Togo.PLoS neglected tropical diseases · 2018Article
- Comparative Genomics Shows That Mycobacterium ulcerans Migration and Expansion Preceded the Rise of Buruli Ulcer in Southeastern Australia.Applied and environmental microbiology · 2018Article
- Perception and incidence of Buruli ulcer in Ogun State, South West Nigeria: intensive epidemiological survey and public health intervention recommended.The Pan African medical journal · 2018Article
- Genotyping Tools forMycobacterial diseases : tuberculosis & leprosy · 2014Article
- Insertion sequence element single nucleotide polymorphism typing provides insights into the population structure and evolution of Mycobacterium ulcerans across Africa.Applied and environmental microbiology · 2014Article
- Article
- Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.PLoS neglected tropical diseases · 2014Article
- Development of a temperature-switch PCR-based SNP typing method for Mycobacterium ulcerans.PLoS neglected tropical diseases · 2012Article
- Sero-epidemiology as a tool to screen populations for exposure to Mycobacterium ulcerans.PLoS neglected tropical diseases · 2012Article
- Treating Mycobacterium ulcerans disease (Buruli ulcer): from surgery to antibiotics, is the pill mightier than the knife?Future microbiology · 2011Review
- Single nucleotide polymorphism typing of Mycobacterium ulcerans reveals focal transmission of buruli ulcer in a highly endemic region of Ghana.PLoS neglected tropical diseases · 2010Article
- Single nucleotide polymorphisms on the road to strain differentiation in Mycobacterium ulcerans.Journal of clinical microbiology · 2009Article
- Lack of insertional-deletional polymorphism in a collection of Mycobacterium ulcerans isolates from Ghanaian Buruli ulcer patients.Journal of clinical microbiology · 2009Article
- Genomic diversity and evolution of Mycobacterium ulcerans revealed by next-generation sequencing.PLoS pathogens · 2009Article
- Sensitivity of PCR targeting Mycobacterium ulcerans by use of fine-needle aspirates for diagnosis of Buruli ulcer.Journal of clinical microbiology · 2009Article
- Independent loss of immunogenic proteins in Mycobacterium ulcerans suggests immune evasion.Clinical and vaccine immunology : CVI · 2008Article
- High rates of fecal carriage of nonenterococcal vanB in both children and adults.Antimicrobial agents and chemotherapy · 2008Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two high-copy-number insertion sequences, IS2404 and IS2606, were recently identified in Mycobacterium ulcerans and were shown by Southern hybridization to possess restriction fragment length polymorphism between strains from different geographic origins. We have designed a simple genotyping method that captures these differences by PCR amplification of the region between adjacent copies of IS2404 and IS2606. We have called this system 2426 PCR. The method is rapid, reproducible, sensitive, and specific for M. ulcerans, and it has confirmed previous studies suggesting a clonal population structure of M. ulcerans within a geographic region. M. ulcerans isolates from Australia, Papua New Guinea, Malaysia, Surinam, Mexico, Japan, China, and several countries in Africa were easily differentiated based on an array of 4 to 14 PCR products ranging in size from 200 to 900 bp. Numerical analysis of the banding patterns suggested a close evolutionary link between M. ulcerans isolates from Africa and southeast Asia. The application of 2426 PCR to total DNA, extracted directly from M. ulcerans-infected tissue specimens without culture, demonstrated the sensitivity and specificity of this method and confirmed for the first time that both animal and human isolates from areas of endemicity in southeast Australia have the same genotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.