ArticleJournal of clinical microbiology2000
Evaluation of diagnostic value and epidemiological implications of PCR for Pneumocystis carinii in different immunosuppressed and immunocompetent patient groups.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 109 citations in OpenAlex.
- Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients (allogeneic SCT excluded): updated guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO).Annals of oncology : official journal of the European Society for Medical Oncology · 2015Guideline
- PCR diagnosis of Pneumocystis pneumonia: a bivariate meta-analysis.Journal of clinical microbiology · 2011Pooled it
- DeterminingDiagnostics (Basel, Switzerland) · 2024Article
- Usefulness of ß-d-Glucan Assay for the First-Line Diagnosis ofJournal of fungi (Basel, Switzerland) · 2022Article
- Performance of a Real Time PCR forJournal of fungi (Basel, Switzerland) · 2022Article
- Middle East respiratory syndrome coronavirus (MERS-CoV) - Surveillance and testing in North England from 2012 to 2019.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Article
- Epidemiology ofFrontiers in cellular and infection microbiology · 2020Article
- Pneumocystis pneumonia: still a serious disease in children.Developmental period medicine · 2019Review
- Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering ofJournal of fungi (Basel, Switzerland) · 2018Article
- Prevalence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Article
- Comparison of quantitative real-time PCR and direct immunofluorescence for the detection of Pneumocystis jirovecii.PloS one · 2017Article
- Detection of Pneumocystis jirovecii by Quantitative PCR To Differentiate Colonization and Pneumonia in Immunocompromised HIV-Positive and HIV-Negative Patients.Journal of clinical microbiology · 2016Article
- Pneumocystis pneumonia in children - the relevance of chemoprophylaxis in different groups of immunocompromised and immunocompetent paediatric patients.Central-European journal of immunology · 2015Article
- Clinical review: Respiratory failure in HIV-infected patients--a changing picture.Critical care (London, England) · 2013Review
- Pneumocystis jirovecii Colonization in Non-HIV-Infected Patients Based on Nested-PCR Detection in Bronchoalveolar Lavage Samples.Iranian journal of public health · 2013Article
- Molecular detection of pneumocystis jirovecii in patients with respiratory tract infections.North American journal of medical sciences · 2012Article
- Article
- PCR-based diagnosis of human fungal infections.Expert review of anti-infective therapy · 2009Review
- Molecular evidence of nosocomial Pneumocystis jirovecii transmission among 16 patients after kidney transplantation.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To evaluate the value of single and nested PCRs for diagnosis of Pneumocystis carinii pneumonia (PCP) in a variety of respiratorily distressed patient groups, 574 respiratory samples from 334 patients (89 human immunodeficiency virus [HIV]-positive patients, 61 transplant recipients, 66 malignancy patients, 34 otherwise immunosuppressed patients, and 84 immunocompetent patients) were prospectively examined by microscopy and single and nested PCRs. The resulting data were correlated with clinical evidence of PCP. Microscopy and single PCR of bronchoalveolar lavage (BAL) specimens from HIV patients were 100% sensitive and specific in detecting PCP, whereas nested PCR, although being 100% sensitive, reached a specificity of only 97.5%. In the three non-HIV immunosuppressed patient groups, both single and nested PCR invariably produced lower positive predictive values than microscopy. Among immunocompetent patients, the positive predictive values of both PCRs were 0%. Therefore, the diagnostic values of the PCR methods tested do not seem to offer any additional advantage compared to that of conventional microscopy for these patient groups. However, nested PCR identified a significant percentage of clinically silent P. carinii colonizations in about 17 to 20% of immunocompetent and immunosuppressed non-HIV patients.
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Registered trials
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