ArticleJournal of clinical microbiology2000
Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in pigs by PCR amplification of the p36 and p46 genes.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 74 citations in OpenAlex.
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- Rapid detection ofFrontiers in cellular and infection microbiology · 2024Article
- Retrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks.Veterinary sciences · 2023Article
- Characteristics ofFrontiers in veterinary science · 2022Article
- Effect of Pregnane X Receptor onAnimals : an open access journal from MDPI · 2021Article
- Attenuation ofFrontiers in veterinary science · 2021Article
- Swine Conjunctivitis Associated with a Novel Mycoplasma Species Closely Related toPathogens (Basel, Switzerland) · 2020Article
- Preparation of Squalene Oil-Based Emulsion Adjuvants Employing a Self-Emulsifying Drug Delivery System and Assessment ofPharmaceutics · 2019Article
- Article
- Associations among Haemophilus parasuis, Mycoplasma hyorhinis, and porcine reproductive and respiratory syndrome virus infections in pigs with polyserositis.The Canadian veterinary journal = La revue veterinaire canadienne · 2015Article
- Multilocus sequence typing of Mycoplasma hyorhinis strains identified by a real-time TaqMan PCR assay.Journal of clinical microbiology · 2014Article
- Activation of EGFR-PI3K-AKT signaling is required for Mycoplasma hyorhinis-promoted gastric cancer cell migration.Cancer cell international · 2014Article
- Comparison of detection procedures of Mycoplasma hyopneumoniae, Mycoplasma hyosynoviae, and Mycoplasma hyorhinis in lungs, tonsils, and synovial fluid of slaughtered pigs and their distributions in Thailand.Tropical animal health and production · 2012Article
- Mycoplasmas hyorhinis in different regions of cuba. diagnosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection.Veterinary journal (London, England : 1997) · 2009Review
- A multiplex PCR to identify porcine mycoplasmas present in broth cultures.Veterinary research communications · 2006Article
- Protein and antigenic variability among Mycoplasma hyopneumoniae strains by SDS-PAGE and immunoblot.Veterinary research communications · 2005Article
- The occurrence of mycoplasmas in the lungs of swine in Gran Canaria (Spain).Veterinary research communications · 2005Article
- Specificity and performance of PCR detection assays for microbial pathogens.Molecular biotechnology · 2004Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of Mycoplasma hyopneumoniae encodes several immunodominant proteins, including a cytosolic protein (p36), three membranous proteins (p46, p65, and p74), and an adhesin (p97). Cross-reactions with M. flocculare and M. hyorhinis reduce the specificity of conventional serological detection methods. However, certain antigenic determinants of the p36 and p46 proteins have been shown to be specific for M. hyopneumoniae. In the present study, pairs of oligonucleotide primers were designed to permit PCR amplification of entire p36 and p46 genes and of internal fragments of these genes. Specific amplicons could be obtained with as low as 0.5 to 50 pg of extracted chromosomal DNA. No amplification product was obtained when testing p36 and p46 primer pairs with genomic DNA or RNA from other mycoplasma species, bacteria, and viruses commonly associated with respiratory diseases in pigs. By using the single p36-PCR method, a positive reaction was demonstrated in 100% (30 of 30) of lungs from pigs that developed typical lesions associated with an M. hyopneumoniae infection, and no false-positive results were detected when 62 apparently normal lungs were tested. On the other hand, with the single p46-PCR method a sensitivity of 86.6% (26 of 30) and a specificity of 96.7% (60 of 62) were obtained in comparison with the necropsy findings. A mixed infection with M. hyorhinis was diagnosed in 13.3% (4 of 30) of the cases by using species-specific primers for the heterologous p37 gene. The sensitivity of the single p36-PCR method for the detection of M. hyopneumoniae, when tested on tracheobronchial swabs, was 100% (20 positive samples), with a specificity of 93.3% (14 of 15 negative samples), compared to the necropsy findings. Both expected amplicons were obtained with 86.6% (26 of 30) positive lungs when p36 and p46 primers were used simultaneously (multiplex PCR) to further increase the specificity of the PCR assay.
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