ArticleThe Journal of cell biology2000
The role of the COOH terminus of Sec2p in the transport of post-Golgi vesicles.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Exploring the consequences of redirecting an exocytic Rab onto endocytic vesicles.Molecular biology of the cell · 2023Article
- Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling.The Journal of cell biology · 2022Article
- Rewiring a Rab regulatory network reveals a possible inhibitory role for the vesicle tether, Uso1.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Regulation of membrane traffic by Rab GEF and GAP cascades.Small GTPases · 2016Review
- The casein kinases Yck1p and Yck2p act in the secretory pathway, in part, by regulating the Rab exchange factor Sec2p.Molecular biology of the cell · 2016Article
- Genome-Wide Transcription Study of Cryptococcus neoformans H99 Clinical Strain versus Environmental Strains.PloS one · 2015Article
- Phosphorylation of the Rab exchange factor Sec2p directs a switch in regulatory binding partners.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis.Molecular biology of the cell · 2011Article
- Hyphal growth in Candida albicans requires the phosphorylation of Sec2 by the Cdc28-Ccn1/Hgc1 kinase.The EMBO journal · 2010Article
- Post-Golgi Sec proteins are required for autophagy in Saccharomyces cerevisiae.Molecular biology of the cell · 2010Article
- Phosphatidylinositol 4-phosphate controls both membrane recruitment and a regulatory switch of the Rab GEF Sec2p.Developmental cell · 2010Article
- A guaninine nucleotide exchange factor is a component of the meiotic spindle pole body in Schizosaccharomyces pombe.Molecular biology of the cell · 2010Article
- The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion.Molecular biology of the cell · 2008Article
- Rabaptin-5-independent membrane targeting and Rab5 activation by Rabex-5 in the cell.Molecular biology of the cell · 2007Article
- Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The rab exchange factor Sec2p reversibly associates with the exocyst.Molecular biology of the cell · 2006Article
- Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast.The EMBO journal · 2004Article
- Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p.The EMBO journal · 2002Article
- Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sec2p is required for the polarized transport of secretory vesicles in S. cerevisiae. The Sec2p NH(2) terminus encodes an exchange factor for the Rab protein Sec4p. Sec2p associates with vesicles and in Sec2p COOH-terminal mutants Sec4p and vesicles no longer accumulate at bud tips. Thus, the Sec2p COOH terminus functions in targeting vesicles, however, the mechanism of function is unknown. We found comparable exchange activity for truncated and full-length Sec2 proteins, implying that the COOH terminus does not alter the exchange rate. Full-length Sec2-GFP, similar to Sec4p, concentrates at bud tips. A COOH-terminal 58-amino acid domain is necessary but not sufficient for localization. Sec2p localization depends on actin, Myo2p and Sec1p, Sec6p, and Sec9p function. Full-length, but not COOH-terminally truncated Sec2 proteins are enriched on membranes. Membrane association of full-length Sec2p is reduced in sec6-4 and sec9-4 backgrounds at 37 degrees C but unaffected at 25 degrees C. Taken together, these data correlate loss of localization of Sec2 proteins with reduced membrane association. In addition, Sec2p membrane attachment is substantially Sec4p independent, supporting the notion that Sec2p interacts with membranes via an unidentified Sec2p receptor, which would increase the accessibility of Sec2p exchange activity for Sec4p.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.