ArticleThe Journal of cell biology2000
Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed, 150 citations in OpenAlex.
- Cytomembrane Trafficking Pathways of Connexin 26, 30, and 43.International journal of molecular sciences · 2023Review
- Exosome secretion kinetics are controlled by temperature.Biophysical journal · 2023Article
- Total Internal Reflection Fluorescence (TIRF) Microscopy.Current protocols · 2022Review
- Spatial and Temporal Control of Protein Secretion with Light.Methods in molecular biology (Clifton, N.J.) · 2022Article
- zapERtrap: A light-regulated ER release system reveals unexpected neuronal trafficking pathways.The Journal of cell biology · 2021Article
- Novel function for AP-1B during cell migration.Molecular biology of the cell · 2020Article
- Architecture and Dynamics of the Neuronal Secretory Network.Annual review of cell and developmental biology · 2019Review
- Novel ecto-tagged integrins reveal their trafficking in live cells.Nature communications · 2017Article
- The exocyst is required for trypanosome invasion and the repair of mechanical plasma membrane wounds.Journal of cell science · 2015Article
- Cellular mechanisms of alpha herpesvirus egress: live cell fluorescence microscopy of pseudorabies virus exocytosis.PLoS pathogens · 2014Article
- Distinct modes of perimembrane TRP channel turnover revealed by TIR-FRAP.Scientific reports · 2014Article
- Tools for controlling protein interactions using light.Current protocols in cell biology · 2014Review
- CLASPs link focal-adhesion-associated microtubule capture to localized exocytosis and adhesion site turnover.Nature cell biology · 2014Article
- Imaging plasma membrane deformations with pTIRFM.Journal of visualized experiments : JoVE · 2014Article
- Four-dimensional live imaging of apical biosynthetic trafficking reveals a post-Golgi sorting role of apical endosomal intermediates.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Membrane tethered delta activates notch and reveals a role for spatio-mechanical regulation of the signaling pathway.Biophysical journal · 2013Article
- Vesicular transport of progeny parvovirus particles through ER and Golgi regulates maturation and cytolysis.PLoS pathogens · 2013Article
- Plasma membrane domains enriched in cortical endoplasmic reticulum function as membrane protein trafficking hubs.Molecular biology of the cell · 2013Article
- A light-triggered protein secretion system.The Journal of cell biology · 2013Article
- Fluorescently-Labeled Estradiol Internalization and Membrane Trafficking in Live N-38 Neuronal Cells Visualized with Total Internal Reflection Fluorescence Microscopy.Journal of steroids & hormonal science · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring the fusion of constitutive traffic with the plasma membrane has remained largely elusive. Ideally, fusion would be monitored with high spatial and temporal resolution. Recently, total internal reflection (TIR) microscopy was used to study regulated exocytosis of fluorescently labeled chromaffin granules. In this technique, only the bottom cellular surface is illuminated by an exponentially decaying evanescent wave of light. We have used a prism type TIR setup with a penetration depth of approximately 50 nm to monitor constitutive fusion of vesicular stomatitis virus glycoprotein tagged with the yellow fluorescent protein. Fusion of single transport containers (TCs) was clearly observed and gave a distinct analytical signature. TCs approached the membrane, appeared to dock, and later rapidly fuse, releasing a bright fluorescent cloud into the membrane. Observation and analysis provided insight about their dynamics, kinetics, and position before and during fusion. Combining TIR and wide-field microscopy allowed us to follow constitutive cargo from the Golgi complex to the cell surface. Our observations include the following: (1) local restrained movement of TCs near the membrane before fusion; (2) apparent anchoring near the cell surface; (3) heterogeneously sized TCs fused either completely; or (4) occasionally larger tubular-vesicular TCs partially fused at their tips.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.