ArticleThe Journal of cell biology2000
Imaging constitutive exocytosis with total internal reflection fluorescence microscopy.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 189 citations in OpenAlex.
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Cytomembrane Trafficking Pathways of Connexin 26, 30, and 43.International journal of molecular sciences · 2023Review
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- Exosome secretion kinetics are controlled by temperature.Biophysical journal · 2023Article
- Spatial organization of lysosomal exocytosis relies on membrane tension gradients.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- PKC-ε regulates vesicle delivery and focal exocytosis for efficient IgG-mediated phagocytosis.Journal of cell science · 2021Article
- pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps.Nature communications · 2021Article
- Article
- CRAC channels regulate astrocyte CaScience signaling · 2019Article
- Single event visualization of unconventional secretion of FGF2.The Journal of cell biology · 2019Article
- Article
- Injured astrocytes are repaired by Synaptotagmin XI-regulated lysosome exocytosis.Cell death and differentiation · 2016Article
- There Is No Simple Model of the Plasma Membrane Organization.Frontiers in cell and developmental biology · 2016Review
- Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.Nature communications · 2015Article
- Surface Trafficking of APP and BACE in Live Cells.Traffic (Copenhagen, Denmark) · 2015Article
- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Review
- Imaging plasma membrane deformations with pTIRFM.Journal of visualized experiments : JoVE · 2014Article
- Imaging cell membrane injury and subcellular processes involved in repair.Journal of visualized experiments : JoVE · 2014Article
- Four-dimensional live imaging of apical biosynthetic trafficking reveals a post-Golgi sorting role of apical endosomal intermediates.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The use of a membrane protein tagged with green fluorescent protein allowed the kinetics of fusion to be followed with a time resolution of 30 frames/s. Quantitative analysis allowed carriers undergoing fusion to be easily distinguished from carriers moving perpendicularly to the plasma membrane. The flattening of the carriers into the plasma membrane is seen as a simultaneous rise in the total, peak, and width of the fluorescence intensity. The duration of this flattening process depends on the size of the carriers, distinguishing small spherical from large tubular carriers. The spread of the membrane protein into the plasma membrane upon fusion is diffusive. Mapping many fusion sites of a single cell reveals that there are no preferred sites for constitutive exocytosis in this system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.