ArticleGenetics2000
Regulation of the yeast INO1 gene. The products of the INO2, INO4 and OPI1 regulatory genes are not required for repression in response to inositol.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- Opi1-mediated transcriptional modulation orchestrates genotoxic stress response in budding yeast.Genetics · 2023Article
- Efficient Production of Glucaric Acid by Engineered Saccharomyces cerevisiae.Applied and environmental microbiology · 2023Article
- Comprehensive Characterization of MutantJournal of microbiology and biotechnology · 2022Article
- Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.International journal of molecular sciences · 2022Article
- Article
- ER-PM membrane contact site regulation by yeast ORPs and membrane stress pathways.PLoS genetics · 2022Article
- Epigenetic memory contributing to the pathogenesis of AKI-to-CKD transition.Frontiers in molecular biosciences · 2022Review
- Establishment and inheritance of epigenetic transcriptional memory.Frontiers in molecular biosciences · 2022Review
- Identification and Characterization of a Novel Basic Helix-Loop-Helix Transcription Factor of Phospholipid Synthesis Regulation inFrontiers in microbiology · 2019Article
- The molecular recognition of phosphatidic acid by an amphipathic helix in Opi1.The Journal of cell biology · 2018Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer.Microbial cell factories · 2018Article
- Role of the inositol pyrophosphate multikinase Kcs1 in Cryptococcus inositol metabolism.Fungal genetics and biology : FG & B · 2018Article
- Transcription factors dynamically control the spatial organization of the yeast genome.Nucleus (Austin, Tex.) · 2016Review
- Strategies to regulate transcription factor-mediated gene positioning and interchromosomal clustering at the nuclear periphery.The Journal of cell biology · 2016Article
- The response to inositol: regulation of glycerolipid metabolism and stress response signaling in yeast.Chemistry and physics of lipids · 2014Review
- High-resolution profiling of stationary-phase survival reveals yeast longevity factors and their genetic interactions.PLoS genetics · 2014Article
- Integrated analysis, transcriptome-lipidome, reveals the effects of INO-level (INO2 and INO4) on lipid metabolism in yeast.BMC systems biology · 2013Article
- Integrated analysis of transcriptome and lipid profiling reveals the co-influences of inositol-choline and Snf1 in controlling lipid biosynthesis in yeast.Molecular genetics and genomics : MGG · 2012Article
- Role for gene looping in intron-mediated enhancement of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Two major inositol transporters and their role in cryptococcal virulence.Eukaryotic cell · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The ino2Delta, ino4Delta, opi1Delta, and sin3Delta mutations all affect expression of INO1, a structural gene for inositol-1-phosphate synthase. These same mutations affect other genes of phospholipid biosynthesis that, like INO1, contain the repeated element UAS(INO) (consensus 5' CATGTGAAAT 3'). In this study, we evaluated the effects of these four mutations, singly and in all possible combinations, on growth and expression of INO1. All strains carrying an ino2Delta or ino4Delta mutation, or both, failed to grow in medium lacking inositol. However, when grown in liquid culture in medium containing limiting amounts of inositol, the opi1Delta ino4Delta strain exhibited a level of INO1 expression comparable to, or higher than, the wild-type strain growing under the same conditions. Furthermore, INO1 expression in the opi1Delta ino4Delta strain was repressed in cells grown in medium fully supplemented with both inositol and choline. Similar results were obtained using the opi1Delta ino2Delta ino4Delta strain. Regulation of INO1 was also observed in the absence of the SIN3 gene product. Therefore, while Opi1p, Sin3p, and the Ino2p/Ino4p complex all affect the overall level of INO1 expression in an antagonistic manner, they do not appear to be responsible for transmitting the signal that leads to repression of INO1 in response to inositol. Various models for Opi1p function were tested and no evidence for binding of Opi1p to UAS(INO), or to Ino2p or Ino4p, was obtained.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.