ArticleGenetics2000
The fission yeast git5 gene encodes a Gbeta subunit required for glucose-triggered adenylate cyclase activation.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Multi-Layered Regulations on the Chromatin Architectures: Establishing the Tight and Specific Responses of Fission YeastBiomolecules · 2022Review
- Use of a Fission Yeast Platform to Identify and Characterize Small Molecule PDE Inhibitors.Frontiers in pharmacology · 2021Review
- A fission yeast platform for heterologous expression of mammalian adenylyl cyclases and high throughput screening.Cellular signalling · 2019Article
- The role of the RACK1 ortholog Cpc2p in modulating pheromone-induced cell cycle arrest in fission yeast.PloS one · 2013Article
- Use of a Schizosaccharomyces pombe PKA-repressible reporter to study cGMP metabolising phosphodiesterases.Cellular signalling · 2011Article
- A fission yeast-based platform for phosphodiesterase inhibitor HTSs and analyses of phosphodiesterase activity.Handbook of experimental pharmacology · 2011Review
- Activated alleles of the Schizosaccharomyces pombe gpa2+ Galpha gene identify residues involved in GDP-GTP exchange.Eukaryotic cell · 2010Article
- The beta subunit of the heterotrimeric G protein triggers the Kluyveromyces lactis pheromone response pathway in the absence of the gamma subunit.Molecular biology of the cell · 2010Article
- G(alpha) and Gbeta proteins regulate the cyclic AMP pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola.Eukaryotic cell · 2009Article
- Article
- The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis.Molecular microbiology · 2007Article
- Propping up our knowledge of G protein signaling pathways: diverse functions of putative noncanonical Gbeta subunits in fungi.Science's STKE : signal transduction knowledge environment · 2007Article
- Cloning the Schizosaccharomyces pombe lys2+ gene and construction of new molecular genetic tools.Current genetics · 2006Article
- Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase.Genetics · 2006Article
- Article
- Article
- Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Except in every detail: comparing and contrasting G-protein signaling in Saccharomyces cerevisiae and Schizosaccharomyces pombe.Eukaryotic cell · 2005Review
- Glucose sensing via the protein kinase A pathway in Schizosaccharomyces pombe.Biochemical Society transactions · 2005Review
- The Gbeta-subunit-encoding gene bpp1 controls cyclic-AMP signaling in Ustilago maydis.Eukaryotic cell · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Fission yeast adenylate cyclase is activated by the gpa2 Galpha subunit of a heterotrimeric guanine-nucleotide binding protein (G protein). We show that the git5 gene, also required for this activation, encodes a Gbeta subunit. In contrast to another study, we show that git5 is not a negative regulator of the gpa1 Galpha involved in the pheromone response pathway. While 43% identical to mammalian Gbeta's, the git5 protein lacks the amino-terminal coiled-coil found in other Gbeta subunits, yet the gene possesses some of the coding capacity for this structure 5' to its ORF. Although both gpa2 (Galpha) and git5 (Gbeta) are required for adenylate cyclase activation, only gpa2 is needed to maintain basal cAMP levels. Strains bearing a git5 disruption are derepressed for fbp1 transcription and sexual development even while growing in a glucose-rich environment, although fbp1 derepression is half that observed in gpa2 deletion strains. Multicopy gpa2 partially suppresses the loss of git5, while the converse is not true. These data suggest that Gbeta is required for activation of adenylate cyclase either by promoting the activation of Galpha or by independently activating adenylate cyclase subsequent to Galpha stimulation as seen in type II mammalian adenylate cyclase activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.