ArticleThe EMBO journal2000
Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 99 citations in OpenAlex.
- Minichromosome Maintenance Proteins Cooperate with LANA during the GJournal of virology · 2019Article
- A novel role for ATR/Rad3 in G1 phase.Scientific reports · 2018Article
- In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Release of condensin from mitotic chromosomes requires the Ran-GTP gradient in the reorganized nucleus.Biology open · 2017Article
- Activation of Gcn2 in response to different stresses.PloS one · 2017Article
- Consequences of abnormal CDK activity in S phase.Cell cycle (Georgetown, Tex.) · 2016Article
- The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage.PloS one · 2015Article
- Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery.Epigenetics & chromatin · 2015Article
- Ectopic centromere nucleation by CENP--a in fission yeast.Genetics · 2014Article
- Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy.Nucleic acids research · 2014Article
- Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast.Yeast (Chichester, England) · 2014Article
- The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.Cell division · 2014Article
- The oxidative stress responsive transcription factor Pap1 confers DNA damage resistance on checkpoint-deficient fission yeast cells.PloS one · 2014Article
- Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis.Molecular biology of the cell · 2013Article
- Induction of a G1-S checkpoint in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Hpz1 modulates the G1-S transition in fission yeast.PloS one · 2012Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.The Journal of biological chemistry · 2011Article
- Article
- Fission yeast receptor of activated C kinase (RACK1) ortholog Cpc2 regulates mitotic commitment through Wee1 kinase.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and washing with a non-ionic detergent. Cell morphology and nuclear structure are preserved in this procedure, allowing structures such as the mitotic spindle to be detected by indirect immunofluorescence. Cell cycle changes in the chromatin association of proteins can therefore be determined from individual cells in asynchronous cultures. We have applied this method to the DNA replication factor mcm4/cdc21, and find that chromatin association occurs during anaphase B, significantly earlier than is the case in budding yeast. Binding of mcm4 to chromatin requires orc1 and cdc18 (homologous to Cdc6 in budding yeast). Release of mcm4 from chromatin occurs during S phase and requires DNA replication. Upon overexpressing cdc18, we show that mcm4 is required for re-replication of the genome in the absence of mitosis and is associated with chromatin in cells undergoing re-replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.