ArticleRNA (New York, N.Y.)2000
Requirements for mini-exon inclusion in potato invertase mRNAs provides evidence for exon-scanning interactions in plants.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- MEPDB: Database of microExons in plants.The New phytologist · 2025Article
- Evidence for Light and Tissue Specific Regulation of Genes Involved in Fructan Metabolism inPlants (Basel, Switzerland) · 2022Article
- Pervasive misannotation of microexons that are evolutionarily conserved and crucial for gene function in plants.Nature communications · 2022Article
- A WheatInternational journal of molecular sciences · 2021Article
- Isoform-specific functions of an evolutionarily conserved 3 bp micro-exon alternatively spliced from another exon in Drosophila homothorax gene.Scientific reports · 2020Article
- Identification and Analysis of Micro-Exon Genes in the Rice Genome.International journal of molecular sciences · 2019Article
- A single-nucleotide exon found in Arabidopsis.Scientific reports · 2015Article
- Alternative splicing of mini-exons in the Arabidopsis leaf rust receptor-like kinase LRK10 genes affects subcellular localisation.Plant cell reports · 2015Article
- RBFOX and PTBP1 proteins regulate the alternative splicing of micro-exons in human brain transcripts.Genome research · 2015Article
- Analysis of splice donor and acceptor function in a novel Ac-based gene trap construct.Planta · 2004Article
- Determinants of plant U12-dependent intron splicing efficiency.The Plant cell · 2004Article
- Computational discovery of internal micro-exons.Genome research · 2003Article
- Full-length messenger RNA sequences greatly improve genome annotation.Genome biology · 2002Article
- Mutational analysis of a plant branchpoint and polypyrimidine tract required for constitutive splicing of a mini-exon.RNA (New York, N.Y.) · 2002Article
- The RNA world in plants: post-transcriptional control III.The Plant cell · 2001Article
- Molecular characterisation of RecQ homologues in Arabidopsis thaliana.Nucleic acids research · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invertases are responsible for the breakdown of sucrose to fructose and glucose. In all but one plant invertase gene, the second exon is only 9 nt in length and encodes three amino acids of a five-amino-acid sequence that is highly conserved in all invertases of plant origin. Sequences responsible for normal splicing (inclusion) of exon 2 have been investigated in vivo using the potato invertase, invGF gene. The upstream intron 1 is required for inclusion whereas the downstream intron 2 is not. Mutations within intron 1 have identified two sequence elements that are needed for inclusion: a putative branchpoint sequence and an adjacent U-rich region. Both are recognized plant intron splicing signals. The branchpoint sequence lies further upstream from the 3' splice site of intron 1 than is normally seen in plant introns. All dicotyledonous plant invertase genes contain this arrangement of sequence elements: a distal branchpoint sequence and adjacent, downstream U-rich region. Intron 1 sequences upstream of the branchpoint and sequences in exons 1, 2, or 3 do not determine inclusion, suggesting that intron or exon splicing enhancer elements seen in vertebrate mini-exon systems are absent. In addition, mutation of the 3' and 5' splice sites flanking the mini-exon cause skipping of the mini-exon, suggesting that both splice sites are required. The branchpoint/U-rich sequence is able to promote splicing of mini-exons of 6, 3, and 1 nt in length and of a chicken cTNT mini-exon of 6 nt. These sequence elements therefore act as a splicing enhancer and appear to function via interactions between factors bound at the branchpoint/U-rich region and at the 5' splice site of intron 2, activating removal of this intron followed by removal of intron 1. This first example of splicing of a plant mini-exon to be analyzed demonstrates that particular arrangement of standard plant intron splicing signals can drive constitutive splicing of a mini-exon.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.