ArticleBritish journal of pharmacology2000
Effects of protein tyrosine kinase inhibitors on voltage-operated calcium channel currents in vascular smooth muscle cells and pp60(c-src) kinase activity.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The vascular Na,K-ATPase: clinical implications in stroke, migraine, and hypertension.Clinical science (London, England : 1979) · 2023Review
- Oxidative Regulation of Vascular CaAntioxidants (Basel, Switzerland) · 2022Review
- A role for focal adhesion kinase in facilitating the contractile responses of murine gastric fundus smooth muscles.The Journal of physiology · 2018Article
- Specialized Functional Diversity and Interactions of the Na,K-ATPase.Frontiers in physiology · 2016Review
- Src kinases regulate de novo actin polymerization during exocytosis in neuroendocrine chromaffin cells.PloS one · 2014Article
- Functional characterization of voltage-dependent Ca(2+) channels in mouse pulmonary arterial smooth muscle cells: divergent effect of ROS.American journal of physiology. Cell physiology · 2013Article
- New mechanisms of pulmonary arterial hypertension: role of Ca²⁺ signaling.American journal of physiology. Heart and circulatory physiology · 2012Review
- The flavonoid scaffold as a template for the design of modulators of the vascular Ca(v) 1.2 channels.British journal of pharmacology · 2011Article
- Multiple kinase pathways regulate voltage-dependent Ca2+ influx and migration in oligodendrocyte precursor cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- The effect of tyrosine nitration of L-type Ca2+ channels on excitation-transcription coupling in colonic inflammation.British journal of pharmacology · 2010Article
- c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta.The Journal of physiology · 2008Article
- Involvement of tyrosine kinase in the hyposmotic stimulation of I Ks in guinea-pig ventricular myocytes.Pflugers Archiv : European journal of physiology · 2008Article
- Signal transduction underlying the control of urinary bladder smooth muscle tone by muscarinic receptors and beta-adrenoceptors.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
- Src family tyrosine kinases mediate contraction of rat isolated tail arteries in response to a hyposmotic stimulus.Journal of hypertension · 2007Article
- Insensitivity of cardiac delayed-rectifier I(Kr) to tyrosine phosphorylation inhibitors and stimulators.British journal of pharmacology · 2006Article
- Src family kinase involvement in rat preglomerular microvascular contractile and [Ca2+]i responses to ANG II.American journal of physiology. Renal physiology · 2005Article
- Inhibition of fast sodium current in rabbit ventricular myocytes by protein tyrosine kinase inhibitors.Pflugers Archiv : European journal of physiology · 2003Article
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3 authors.
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Abstract
1. Tyrosine kinases have been proposed as regulators of voltage-operated calcium channels. The effects of a range of structurally different inhibitors of protein tyrosine kinases (PTK) were examined on voltage-operated calcium channel currents (I(Ba)) and pp60(c-src) kinase (c-src) activity in vitro. 2. I(Ba) was measured in single myocytes isolated from rabbit ear artery by conventional whole cell voltage-clamp techniques. The activity of purified human c-src was measured in vitro using a non-radioactive assay. 3. Bath application of tyrphostin-23 and genistein (non-selective PTK inhibitors), bistyrphostin (a receptor-PTK-selective inhibitor) and PP1 (a src family-selective inhibitor) inhibited I(Ba) in a concentration-dependent manner over a range of test membrane potentials. Intracellular application of peptide-A, a peptide inhibitor of c-src also inhibited currents. Inhibitor potency series against I(Ba) was PP1 > genistein > tyrphostin 23 > bistyrphostin. 4. Tyrphostin-23, genistein, PP1, and peptide-A shifted the steady-state inactivation curves in a hyperpolarized direction without altering their slope. The inhibitors had no significant effects on I(Ba) activation calculated from current-voltage relationships. 5. The agents inhibited c-src activity in a concentration-dependent manner. The order of potency was PP1 > genistein > peptide-A > tyrphostin-23 > bistyrphostin. The IC(50) for inhibition of c-src activity was similar to the IC(50) for inhibition of I(Ba) in all cases. 6. Western blot analysis with a specific antibody to c-src showed the presence of this cytoplasmic tyrosine kinase in rabbit ear artery cells. 7. A range of structurally dissimilar inhibitors of PTKs inhibit I(Ba) and c-src activity with similar potency. These data provide further evidence implicating endogenous c-src in the modulation of L-type calcium channels in vascular smooth muscle cells.
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