ArticleAmerican journal of human genetics2000
Comparative genomic hybridization in combination with flow cytometry improves results of cytogenetic analysis of spontaneous abortions.
Article in American journal of human genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 159 citations in OpenAlex.
- Array Comparative Genomic Hybridization Analysis of Products of Conception in Recurrent Pregnancy Loss for specific anomalies detected by USG.Reproduction & fertility · 2026Article
- Preimplantation genetic testing for aneuploidy on previously cryopreserved unbiopsied blastocysts: a cohort study in women with IVF pregnancy loss.Reproductive biology and endocrinology : RB&E · 2025Observational
- Simple Sequence Repeats (SSRs) and Telomeric Analysis in Somatic Organs of Reproductive and Non-Reproductive Castes of TermiteBiology · 2025Article
- The Landscape of Point Mutations in Human Protein Coding Genes Leading to Pregnancy Loss.International journal of molecular sciences · 2023Review
- Article
- Comparative clinical genetic testing in spontaneous miscarriage: Insights from a study in Southern Chinese women.Journal of cellular and molecular medicine · 2021Article
- Identifying the causes of recurrent pregnancy loss in consanguineous couples using whole exome sequencing on the products of miscarriage with no chromosomal abnormalities.Scientific reports · 2021Article
- Genetic analysis of products of conception. Should we abandon classic karyotyping methodology?Einstein (Sao Paulo, Brazil) · 2021Article
- Chromosomal Aneuploidy Associated With Clinical Characteristics of Pregnancy Loss.Frontiers in genetics · 2021Article
- Detection of chromosomal abnormalities in spontaneous miscarriage by low‑coverage next‑generation sequencing.Molecular medicine reports · 2020Article
- Chromosomal aberrations in pregnancy and fetal loss: Insight on the effect of consanguinity, review of 1625 cases.Molecular genetics & genomic medicine · 2019Article
- Embryoscopy and karyotype findings of repeated miscarriages in recurrent pregnancy loss and spontaneous pregnancy loss.Journal of assisted reproduction and genetics · 2018Article
- Chromosomal abnormalities in products of conception of first-trimester miscarriages detected by conventional cytogenetic analysis: a review of 1000 cases.Journal of assisted reproduction and genetics · 2018Article
- Submicroscopic chromosomal imbalances contribute to early abortion.Molecular cytogenetics · 2018Article
- [Single nucleotide polymorphism-array in genetic analysis of chorionic villi from early spontaneous miscarriages].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2017Article
- Comprehensive genetic analysis of pregnancy loss by chromosomal microarrays: outcomes, benefits, and challenges.Genetics in medicine : official journal of the American College of Medical Genetics · 2017Article
- Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles.Molecular medicine reports · 2016Article
- Aneuploidy in Early Miscarriage and its Related Factors.Chinese medical journal · 2015Article
- Molecular genetic studies of complete hydatidiform moles.Translational pediatrics · 2015Review
- 1(st) trimester miscarriage: four decades of study.Translational pediatrics · 2015Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
More than 50% of spontaneous abortions (SAs) have abnormal chromosomes; the most common abnormalities are trisomy, sex chromosome monosomy, and polyploidy. Conventional cytogenetic analysis of SAs depends on tissue culturing and is associated with a significant tissue culture failure rate and contamination by maternally derived cells. Comparative genomic hybridization (CGH), in combination with flow cytometry (FCM), can detect numerical and unbalanced structural chromosomal abnormalities associated with SAs while avoiding the technical problems associated with tissue culture. Routine cytogenetic and CGH analysis was performed independently on tissue from 301 SAs. Samples shown to be chromosomally balanced by CGH were analyzed by FCM to determine ploidy. Of 253 samples successfully analyzed by both approaches, there was an absolute correlation of results in 235 (92.8%). Of the 18 cases with discrepancies between cytogenetic and CGH/FCM results, an explanation could be found in 17. Twelve samples produced a 46,XX karyotype by cytogenetics, whereas CGH/FCM demonstrated aneuploidy/polyploidy or a male genome, indicating maternal contamination of the tissue cultures. In two cases, where tetraploidy was demonstrated by cytogenetics and diploidy by FCM, tissue culture artifact is implied. In three cases, CGH demonstrated an aneuploidy, and cytogenetics demonstrated hypertriploidy. In one unexplainable case, aneuploidy demonstrated by CGH could not be detected by repeat CGH analysis, conventional cytogenetic, or FISH analysis. These results demonstrate that CGH supplemented with FCM can readily identify chromosomal abnormalities associated with SAs and, by avoiding maternal contamination and tissue culture artifacts, can do so with a lower failure rate and more accuracy than conventional cytogenetic analysis.
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