ArticleProceedings of the National Academy of Sciences of the United States of America2000
Direct interaction between synaptotagmin and the intracellular loop I-II of neuronal voltage-sensitive sodium channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Common themes in evoked ion channel translocation in neuroplastic and homeostatic plasticity.Frontiers in pharmacology · 2026Review
- Selective inhibition of gamma aminobutyric acid release from mouse hippocampal interneurone subtypes by the volatile anaesthetic isoflurane.British journal of anaesthesia · 2021Article
- Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti.PloS one · 2019Article
- CRMP2-Neurofibromin Interface Drives NF1-related Pain.Neuroscience · 2018Article
- Mapping protein interactions of sodium channel NaThe EMBO journal · 2018Article
- Sodium channel subtypes are differentially localized to pre- and post-synaptic sites in rat hippocampus.The Journal of comparative neurology · 2017Article
- Proteomic analysis of native cerebellar iFGF14 complexes.Channels (Austin, Tex.) · 2016Article
- Quantitative proteomics reveals protein-protein interactions with fibroblast growth factor 12 as a component of the voltage-gated sodium channel 1.2 (nav1.2) macromolecular complex in Mammalian brain.Molecular & cellular proteomics : MCP · 2015Article
- Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.Molecular brain · 2014Article
- GPCR mediated regulation of synaptic transmission.Progress in neurobiology · 2012Review
- Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Enhancement of sodium current in NG108-15 cells during neural differentiation is mainly due to an increase in NaV1.7 expression.Neurochemical research · 2007Article
- Patterning of endocytic vesicles and its control by voltage-gated Na+ channel activity in rat prostate cancer cells: fractal analyses.European biophysics journal : EBJ · 2004Article
- Voltage-gated Na+ channels: multiplicity of expression, plasticity, functional implications and pathophysiological aspects.European biophysics journal : EBJ · 2004Review
- Functional maturation of nicotinic acetylcholine receptors as an indicator of murine muscular differentiation in a new nerve-muscle co-culture system.Pflugers Archiv : European journal of physiology · 2003Article
- Role of synaptotagmin in Ca2+-triggered exocytosis.The Biochemical journal · 2002Review
- Identification of an axonal determinant in the C-terminus of the sodium channel Na(v)1.2.The EMBO journal · 2001Article
- A novel SCN1A mutation associated with generalized epilepsy with febrile seizures plus--and prevalence of variants in patients with epilepsy.American journal of human genetics · 2001Article
- Direct interaction of a brain voltage-gated K+ channel with syntaxin 1A: functional impact on channel gating.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptotagmin, a synaptic vesicle protein involved in Ca(2+)-regulated exocytosis, displayed direct high affinity interaction with neuronal sodium channels. Monoclonal antibodies directed against synaptotagmins I and II adsorbed in a concentration-dependent and -specific manner [(3)H]saxitoxin prelabeled sodium channels extracted with detergent from nerve endings. Conversely, co-immunoprecipitation of synaptotagmin was achieved by antibodies against sodium channel subunits. Consistent with the co-immunoprecipitation assays, solubilized [(3)H]saxitoxin-prelabeled sodium channels were trapped on immobilized maltose binding protein (MBP)-synaptotagmin I. In vitro recombinant protein assays were employed to identify the interaction site of synaptotagmin I, which was located on the cytoplasmic loop between domains I and II of the sodium channel alphaIIA subunit. The co-immunoprecipitated synaptotagmin-sodium channel complexes were found to be Ca(2+)-dependent; this effect was mimicked by Ba(2+) and Sr(2+) but not Mg(2+). Finally the complex was shown to be distinct from the synaptotagmin-SNARE protein complex that can selectively interact with presynaptic calcium channels (N and P/Q types). Thus, our findings demonstrate an unexpected and direct interaction between sodium channels and synaptotagmin. The Ca(2+)-regulated association between sodium channels and a protein implicated in vesicular fusion may have intriguing consequences for the establishment and regulation of neuronal excitability.
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