Evidence map›Paper›PMID 10737729›Full record

ReviewExperimental & applied acarology1999

Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review.

O A Sparagano, M T Allsopp, R A Mank, S G Rijpkema, J V Figueroa, F Jongejan

Abstract readReview
PubMed Publisher
In one paragraph

Review in Experimental & applied acarology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Transovarial Transmission ofPathogens (Basel, Switzerland) · 2023
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Challenges in Tick-Borne Pathogen Detection: The Case forPathogens (Basel, Switzerland) · 2021
    Review
  10. Geographical Distribution ofPathogens (Basel, Switzerland) · 2020
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. First record of Borrelia burgdorferi B31 strain in Dermacentor nitens ticks in the northern region of Parana (Brazil).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

O A SparaganoDivision of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. O.A.E.Sparagano@hw.ac.uk
M T Allsopp
R A Mank
S G Rijpkema
J V Figueroa
F Jongejan
Utrecht University · NLNational Institute for Biological Standards and Control · GBOnderstepoort Veterinary Institute · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ticks play an important role in human and veterinary medicine, in particular due to their ability to transmit a wide spectrum of pathogenic micro-organisms of protozoal, rickettsial, bacterial and viral origin. Pathogens in ticks can be identified by conventional methods such as indirect immunofluorescence, isolation in cell culture or by using histological staining techniques. However, the advent of the polymerase chain reaction (PCR) has resulted in tremendous improvements in the specific and sensitive detection of pathogen DNA in ticks. In this paper, literature on DNA extraction methods, PCR protocols, primers and probes, which are in use for the successful detection and identification of pathogens in ticks, are critically reviewed. Some recommendations are also given towards the end of this review.

Indexed as

AnaplasmaAnimalsArachnid VectorsBabesiaBorreliaDNA, BacterialDNA PrimersDNA, ProtozoanDNA, ViralEhrlichiaEhrlichia ruminantiumEncephalitis Viruses, Tick-BorneNairovirusPolymerase Chain ReactionTheileriaTick-Borne DiseasesDNA, BacterialDNA PrimersDNA, ProtozoanDNA, Viral

Identifiers

PMID10737729
OpenAlexW54654328

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.