ArticlePlant molecular biology1999
Expression of fungal thermotolerant endo-1,4-beta-glucanase in transgenic barley seeds during germination.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 66 citations in OpenAlex.
- Barley's Second Spring as A Model Organism for Chloroplast Research.Plants (Basel, Switzerland) · 2020Review
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
- Improved recombinant cellulase expression in chloroplast of tobacco through promoter engineering and 5' amplification promoting sequence.Plant molecular biology · 2013Article
- Article
- Barley genomics: An overview.International journal of plant genomics · 2008Article
- Heterologous expression of Vitreoscilla haemoglobin in barley (Hordeum vulgare).Plant cell reports · 2007Article
- Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice.Transgenic research · 2006Article
- Selectable marker-free transgenic barley producing a high level of cellulase (1,4-beta-glucanase) in developing grains.Plant cell reports · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The malting quality of two barley cultivars, Kymppi and Golden Promise, was modified to better meet the requirements of the brewing process. The egl1 gene, coding for fungal thermotolerant endo-1,4-beta-glucanase (EGI, cellulase), was transferred to the cultivars using particle bombardment, and transgenic plants were regenerated on bialaphos selection. Integration of the egl1 gene was confirmed by Southern blot hybridization. The transgenic seeds were screened for the expression of the heterologous EGI. Under the high-pI alpha-amylase promoter, the egl1 gene was expressed during germination. The heterologous enzyme was thermotolerant at 65 degrees C for 2 h, thus being suitable for mashing conditions. The amount of heterologous EGI produced by the seeds (ca. 0.025% of soluble seed protein), has been shown to be sufficient to reduce wort viscosity by decreasing the soluble beta-glucan content. A decrease in the soluble beta-glucan content in the wort improves the filtration rate of beer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.