ArticlePlant molecular biology1999
Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Evolution of folate biosynthesis and metabolism across algae and land plant lineages.Scientific reports · 2019Article
- Dihydrofolate Reductase/Thymidylate Synthase Fine-Tunes the Folate Status and Controls Redox Homeostasis in Plants.The Plant cell · 2017Article
- Folates in Plants: Research Advances and Progress in Crop Biofortification.Frontiers in chemistry · 2017Review
- Distinctive features and differential regulation of the DRTS genes of Arabidopsis thaliana.PloS one · 2017Article
- Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels.BMC plant biology · 2015Article
- Study on Folate Binding Domain of Dihydrofolate Reductase in Different Plant species and Human beings.Bioinformation · 2014Article
- The folylpolyglutamate synthetase plastidial isoform is required for postembryonic root development in Arabidopsis.Plant physiology · 2011Article
- Cloning, expression, and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: inhibitory effect of antifolates on its catalytic activity and parasite proliferation.Antimicrobial agents and chemotherapy · 2008Article
- One-carbon metabolism in plants. Regulation of tetrahydrofolate synthesis during germination and seedling development.Plant physiology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A bifunctional gene (ZmDHFR-TS) encoding dihydrofolate reductase (DHFR) and thymidylate synthase (TS) was cloned from a Zea mays cDNA library. Both of these enzymes are involved in nucleotide biosynthesis, specifically in the formation of thymidine monophosphate (TMP). Comparison of the deduced amino acid sequence with DHFR-TS sequences from three other plant sources revealed over 75% similarity and motifs typical of DHFR-TS proteins. Two copies of the gene were mapped to chromosomes 2 and 4. This represents the first DHFR-TS gene cloned from a monocotyledonous plant. Expression of ZmDHFR-TS was examined in developing kernels and various tissues of maize by RNA gel blot hybridization analysis in order to determine the relationship between expression of this gene and DNA synthesis. RNA transcripts for ZmDHFR-TS accumulated to high levels in developing maize kernels when endosperm cells were undergoing endoreduplication and cell division. Meristematic maize tissues had high levels of ZmDHFR-TS mRNA, but transcripts were barely detectable in RNA isolated from the root elongation zone and from mature leaf tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.