Evidence map›Paper›PMID 10735886›Full record

ArticleJournal of bacteriology2000

Identification of an operon required for ferrichrome iron utilization in Vibrio cholerae.

M B Rogers, J A Sexton, G J DeCastro, S B Calderwood

Open access · greenAbstract readComparative Study
In one paragraph

Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 58 citations in OpenAlex.

  1. The role of solute binding proteins in signal transduction.Computational and structural biotechnology journal · 2021
    Review
  2. Siderophore piracy enhancesMicrobiology (Reading, England) · 2020
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Vibrio cholerae VciB Mediates Iron Reduction.Journal of bacteriology · 2017
    Article
  8. Iron Acquisition Strategies ofFrontiers in cellular and infection microbiology · 2017
    Review
  9. Article
  10. Vibrio Iron Transport: Evolutionary Adaptation to Life in Multiple Environments.Microbiology and molecular biology reviews : MMBR · 2016
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

M B RogersInfectious Disease Division, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
J A Sexton
G J DeCastro
S B Calderwood
Massachusetts General Hospital · USHarvard University · US

Funding

IRON UPTAKE BY VIBRIO CHOLERAE AND ROLE IN PATHOGENESISF32DK009651 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI ROGERS, MARC B · 1997 to 1998
–
IRON-REGULATED VIRULENCE GENES OF VIBRIO CHOLERAER01AI034968 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI CALDERWOOD, STEPHEN B. · 1994 to 1998
–
NIAID NIH HHS R01AI34968NIDDK NIH HHS F32 DK009651NIDDK NIH HHS F32DK09651
6 · The paper itself

Abstract

Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of which was deficient in the ability to utilize ferrichrome as a sole iron source for growth in a plate bioassay and had an insertion in genes encoding products homologous to Escherichia coli FhuA or FhuD. Expression of the gene fusions was independent of IrgB but regulated by Fur. We report here a map of the operon and the predicted amino acid sequence of FhuA, based on the nucleotide sequence. Unlike those of the E. coli fhu operon, the V. cholerae ferrichrome utilization genes are located adjacent and opposite in orientation to a gene encoding an ATP-binding cassette transporter homolog, but this gene, if disrupted, does not affect the utilization of ferrichrome in vitro.

Indexed as

Escherichia coli ProteinsGenes, BacterialOperonAlkaline PhosphataseAmino Acid SequenceBacterial Outer Membrane ProteinsFerrichromeIronMolecular Sequence DataMutagenesis, InsertionalReceptors, VirusRecombinant Fusion ProteinsSequence Homology, Amino AcidVibrio choleraeAlkaline PhosphataseBacterial Outer Membrane ProteinsEscherichia coli ProteinsFerrichromeFhuA protein, E coliIronReceptors, VirusRecombinant Fusion Proteins

Identifiers

PMID10735886
PMCPMC111292
OpenAlexW2113480767

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.