ArticleJournal of bacteriology2000
A study of AroP-PheP chimeric proteins and identification of a residue involved in tryptophan transport.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- The Enigmatic Conserved Q134-F135-N137 Triad in SARS-CoV-2 Spike Protein: A Conformational Transducer?Biomolecules · 2026Review
- Tripartite Loops Reverse Antibiotic Resistance.Molecular biology and evolution · 2025Article
- Identification and cultivation of anaerobic bacterial scavengers of dead cells.The ISME journal · 2023Article
- Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria.Nature biotechnology · 2018Article
- A L-lysine transporter of high stereoselectivity of the amino acid-polyamine-organocation (APC) superfamily: production, functional characterization, and structure modeling.The Journal of biological chemistry · 2014Article
- Identification of a receptor subunit and putative ligand-binding residues involved in the Bacillus megaterium QM B1551 spore germination response to glucose.Journal of bacteriology · 2010Article
- Aromatic amino acid auxotrophs constructed by recombinant marker exchange in Methylophilus methylotrophus AS1 cells expressing the aroP-encoded transporter of Escherichia coli.Applied and environmental microbiology · 2010Article
- Structure and mechanism of a Na+-independent amino acid transporter.Science (New York, N.Y.) · 2009Article
- YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity.Journal of bacteriology · 2007Article
- Identification of the LIV-I/LS system as the third phenylalanine transporter in Escherichia coli K-12.Journal of bacteriology · 2004Article
- Putative interhelical interactions within the PheP protein revealed by second-site suppressor analysis.Journal of bacteriology · 2003Article
- Study of second-site suppression in the pheP gene for the phenylalanine transporter of Escherichia coli.Journal of bacteriology · 2002Article
- Substrate specificity of nickel/cobalt permeases: insights from mutants altered in transmembrane domains I and II.Journal of bacteriology · 2002Article
- Functional analysis of the Erwinia herbicola tutB gene and its product.Journal of bacteriology · 2002Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo recombination has been used to make a series of AroP-PheP chimeric proteins. Analysis of their respective substrate profiles and activities has identified a small region within span III of AroP which can confer on a predominantly PheP protein the ability to transport tryptophan. Site-directed mutagenesis of the AroP-PheP chimera, PheP, and AroP has established that a key residue involved in tryptophan transport is tyrosine at position 103 in AroP. Phenylalanine is the residue at the corresponding position in PheP. The use of PheP-specific antisera has shown that the inability of certain chimeras to transport any of the aromatic amino acids is not a result of instability or a failure to be inserted into the membrane. Site-directed mutagenesis has identified two significant AroP-specific residues, alanine 107 and valine 114, which are the direct cause of loss of transport activity in chimeras such as A152P. These residues replace a glycine and an alanine in PheP and flank a highly conserved glutamate at position 110. Some suggestions are made as to the possible functions of these residues in the tertiary structure of the proteins.
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