ArticleJournal of bacteriology2000
Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 97 citations in OpenAlex.
- Article
- Phylogenetic Analyses of Microbial Hydrolytic Dehalogenases Reveal Polyphyletic Origin.Indian journal of microbiology · 2022Article
- Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds.Frontiers in microbiology · 2018Review
- The TetR family of regulators.Microbiology and molecular biology reviews : MMBR · 2013Review
- NemR is a bleach-sensing transcription factor.The Journal of biological chemistry · 2013Article
- Why are chlorinated pollutants so difficult to degrade aerobically? Redox stress limits 1,3-dichloroprop-1-ene metabolism by Pseudomonas pavonaceae.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- First description of natural and experimental conjugation between Mycobacteria mediated by a linear plasmid.PloS one · 2012Article
- Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A.Applied microbiology and biotechnology · 2012Article
- Facilitation of bacterial adaptation to chlorothalonil-contaminated sites by horizontal transfer of the chlorothalonil hydrolytic dehalogenase gene.Applied and environmental microbiology · 2011Article
- The role of laterally transferred genes in adaptive evolution.BMC evolutionary biology · 2007Article
- ISCR elements: novel gene-capturing systems of the 21st century?Microbiology and molecular biology reviews : MMBR · 2006Review
- Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases.Applied and environmental microbiology · 2005Article
- The TetR family of transcriptional repressors.Microbiology and molecular biology reviews : MMBR · 2005Review
- Indications for acquisition of reductive dehalogenase genes through horizontal gene transfer by Dehalococcoides ethenogenes strain 195.Applied and environmental microbiology · 2005Article
- Biodegradation, biotransformation, and biocatalysis (b3).Applied and environmental microbiology · 2002Review
- trans-3-Chloroacrylic acid dehalogenase from Pseudomonas pavonaceae 170 shares structural and mechanistic similarities with 4-oxalocrotonate tautomerase.Journal of bacteriology · 2001Article
- Genetic diversity among 3-chloroaniline- and aniline-degrading strains of the Comamonadaceae.Applied and environmental microbiology · 2001Article
- Haloalkane-utilizing Rhodococcus strains isolated from geographically distinct locations possess a highly conserved gene cluster encoding haloalkane catabolism.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The haloalkane-degrading bacteria Rhodococcus rhodochrous NCIMB13064, Pseudomonas pavonaceae 170, and Mycobacterium sp. strain GP1 share a highly conserved haloalkane dehalogenase gene (dhaA). Here, we describe the extent of the conserved dhaA segments in these three phylogenetically distinct bacteria and an analysis of their flanking sequences. The dhaA gene of the 1-chlorobutane-degrading strain NCIMB13064 was found to reside within a 1-chlorobutane catabolic gene cluster, which also encodes a putative invertase (invA), a regulatory protein (dhaR), an alcohol dehydrogenase (adhA), and an aldehyde dehydrogenase (aldA). The latter two enzymes may catalyze the oxidative conversion of n-butanol, the hydrolytic product of 1-chlorobutane, to n-butyric acid, a growth substrate for many bacteria. The activity of the dhaR gene product was analyzed in Pseudomonas sp. strain GJ1, in which it appeared to function as a repressor of dhaA expression. The 1,2-dibromoethane-degrading strain GP1 contained a conserved DNA segment of 2.7 kb, which included dhaR, dhaA, and part of invA. A 12-nucleotide deletion in dhaR led to constitutive expression of dhaA in strain GP1, in contrast to the inducible expression of dhaA in strain NCIMB13064. The 1, 3-dichloropropene-degrading strain 170 possessed a conserved DNA segment of 1.3 kb harboring little more than the coding region of the dhaA gene. In strains 170 and GP1, a putative integrase gene was found next to the conserved dhaA segment, which suggests that integration events were responsible for the acquisition of these DNA segments. The data indicate that horizontal gene transfer and integrase-dependent gene acquisition were the key mechanisms for the evolution of catabolic pathways for the man-made chemicals 1, 3-dichloropropene and 1,2-dibromoethane.
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