ArticleJournal of bacteriology2000
Type IV pilus genes pilA and pilC of Pseudomonas stutzeri are required for natural genetic transformation, and pilA can be replaced by corresponding genes from nontransformable species.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Beyond the Usual Suspects: EmergingInternational journal of molecular sciences · 2026Review
- From isotopically labeled DNA to fluorescently labeled dynamic pili: building a mechanistic model of DNA transport to the cytoplasmic membrane.Microbiology and molecular biology reviews : MMBR · 2024Review
- Genomic Characterization of Carbapenem-Resistant Bacteria from Beef Cattle Feedlots.Antibiotics (Basel, Switzerland) · 2023Article
- Comparative genomics ofFrontiers in microbiology · 2023Article
- Retraction ATPase Motors from Three Orthologous Type IVa Pilus Systems Support Promiscuous Retraction of the Vibrio cholerae Competence Pilus.Journal of bacteriology · 2022Article
- Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation.Extremophiles : life under extreme conditions · 2021Review
- Evolutionary Plasticity of AmrZ Regulation inmSphere · 2018Article
- Structure of the competence pilus major pilin ComGC inThe Journal of biological chemistry · 2017Article
- Complete genome sequence ofStandards in genomic sciences · 2017Article
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- Natural competence and the evolution of DNA uptake specificity.Journal of bacteriology · 2014Review
- Structure and function of PilQ, a secretin of the DNA transporter from the thermophilic bacterium Thermus thermophilus HB27.The Journal of biological chemistry · 2011Article
- The accessory genome of Pseudomonas aeruginosa.Microbiology and molecular biology reviews : MMBR · 2010Review
- Gene sequences of the pil operon reveal relationships between symbiotic strains of Vibrio fischeri.International journal of systematic and evolutionary microbiology · 2008Article
- Pseudomonas aeruginosa Type IV pilus expression in Neisseria gonorrhoeae: effects of pilin subunit composition on function and organelle dynamics.Journal of bacteriology · 2007Article
- Functional analysis of PilT from the toxic cyanobacterium Microcystis aeruginosa PCC 7806.Journal of bacteriology · 2007Article
- Biology of Pseudomonas stutzeri.Microbiology and molecular biology reviews : MMBR · 2006Review
- Identification of pilus-like structures and genes in Microcystis aeruginosa PCC7806.Applied and environmental microbiology · 2005Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas stutzeri lives in terrestrial and aquatic habitats and is capable of natural genetic transformation. After transposon mutagenesis, transformation-deficient mutants were isolated from a P. stutzeri JM300 strain. In one of them a gene which coded for a protein with 75% amino acid sequence identity to PilC of Pseudomonas aeruginosa, an accessory protein for type IV pilus biogenesis, was inactivated. The presence of type IV pili was demonstrated by susceptibility to the type IV pilus-dependent phage PO4, by occurrence of twitching motility, and by electron microscopy. The pilC mutant had no pili and was defective in twitching motility. Further sequencing revealed that pilC is clustered in an operon with genes homologous to pilB and pilD of P. aeruginosa, which are also involved in pilus formation. Next to these genes but transcribed in the opposite orientation a pilA gene encoding a protein with high amino acid sequence identity to pilin, the structural component of type IV pili, was identified. Insertional inactivation of pilA abolished pilus formation, PO4 plating, twitching motility, and natural transformation. The amounts of (3)H-labeled P. stutzeri DNA that were bound to competent parental cells and taken up were strongly reduced in the pilC and pilA mutants. Remarkably, the cloned pilA genes from nontransformable organisms like Dichelobacter nodosus and the PAK and PAO strains of P. aeruginosa fully restored pilus formation and transformability of the P. stutzeri pilA mutant (along with PO4 plating and twitching motility). It is concluded that the type IV pili of the soil bacterium P. stutzeri function in DNA uptake for transformation and that their role in this process is not confined to the species-specific pilin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.