ArticleBiophysical journal2000
Effects of temperature on calcium-sensitive fluorescent probes.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- A Microdroplet Fluorescence Method for the Determination of Nitrite in Water Using a Hydrophobic Cellulose Substrate Platform.ACS omega · 2026Article
- Cold- and TRPM8-dependent shaking in mammals and birds.Communications biology · 2025Article
- Abiotic Stress-Induced Chloroplast and Cytosolic CaPlant, cell & environment · 2025Article
- Article
- Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets.PloS one · 2024Article
- ThermoCyte: an inexpensive open-source temperature control system forRoyal Society open science · 2023Article
- Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets.bioRxiv : the preprint server for biology · 2023Article
- STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons.The EMBO journal · 2023Article
- Bidirectional modulation of evoked synaptic transmission by pulsed infrared light.Scientific reports · 2022Article
- Intracellular CaJournal of cardiovascular translational research · 2022Article
- Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo.Scientific reports · 2021Article
- Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems.RSC advances · 2021Article
- Ageing-associated increase in SGLT2 disrupts mitochondrial/sarcoplasmic reticulum CaJournal of cellular and molecular medicine · 2020Article
- Single-Cell Optogenetic Control of Calcium Signaling with a High-Density Micro-LED Array.iScience · 2019Article
- Barium Chemosensors with Dry-Phase Fluorescence for Neutrinoless Double Beta Decay.Scientific reports · 2019Article
- Pulsed infrared releases CaJournal of neurophysiology · 2018Article
- Neural basis of trigeminal chemo- and thermonociception in brown treesnakes, Boiga irregularis (Squamata: Colubridae).Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2018Article
- Article
- The TRPM2 channel is a hypothalamic heat sensor that limits fever and can drive hypothermia.Science (New York, N.Y.) · 2016Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The effect of temperature on the binding equilibria of calcium-sensing dyes has been extensively studied, but there are also important temperature-related changes in the photophysics of the dyes that have been largely ignored. We conducted a systematic study of thermal effects on five calcium-sensing dyes under calcium-saturated and calcium-free conditions. Quin-2, chlortetracycline, calcium green dextran, Indo-1, and Fura-2 all show temperature-dependent effects on fluorescence in all or part of the range tested (5-40 degrees C). Specifically, the intensity of the single-wavelength dyes increased at low temperature. The ratiometric dyes, because of variable effects at the two wavelengths, showed, in general, a reduction in the fluorescence ratio as temperature decreased. Changes in viscosity, pH, oxygen quenching, or fluorescence maxima could not fully explain the effects of temperature on fluorescence. The excited-state lifetimes of the dyes were determined, in both the presence and absence of calcium, using multifrequency phase-modulation fluorimetry. In most cases, low temperature led to prolonged fluorescence lifetimes. The increase in lifetimes at reduced temperature is probably largely responsible for the effects of temperature on the physical properties of the calcium-sensing dyes. Clearly, these temperature effects can influence reported calcium concentrations and must therefore be taken into consideration during any investigation involving variable temperatures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.