ArticleBiophysical journal2000
Effects of cytoplasmic and luminal pH on Ca(2+) release channels from rabbit skeletal muscle.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- β-alanine supplementation improves in-vivo fresh and fatigued skeletal muscle relaxation speed.European journal of applied physiology · 2017Trial
- Muscle cramps as disorders of impaired termination of contraction: An integrated neurophysiological framework.Physiological reports · 2026Review
- Lactic acidosis: implications for human exercise performance.European journal of applied physiology · 2025Review
- The Cardiac Ryanodine Receptor Provides a Suitable Pathway for the Rapid Transport of Zinc (ZnCells · 2022Article
- Molecular Basis for Exercise-Induced Fatigue: The Importance of Strictly Controlled Cellular CaCold Spring Harbor perspectives in medicine · 2018Review
- The CaMolecular bioSystems · 2017Review
- In vivo CaPhysiological reports · 2017Article
- Bilayer measurement of endoplasmic reticulum Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Coupled gating of skeletal muscle ryanodine receptors is modulated by Ca2+, Mg2+, and ATP.American journal of physiology. Cell physiology · 2012Article
- Alterations in the calcium homeostasis of skeletal muscle from postmyocardial infarcted rats.Pflugers Archiv : European journal of physiology · 2007Article
- The conformation of calsequestrin determines its ability to regulate skeletal ryanodine receptors.Biophysical journal · 2006Article
- Coupled calcium release channels and their regulation by luminal and cytosolic ions.European biophysics journal : EBJ · 2005Review
- Luminal Ca2+-regulated Mg2+ inhibition of skeletal RyRs reconstituted as isolated channels or coupled clusters.The Journal of general physiology · 2004Article
- Suppression of calcium sparks in rat ventricular myocytes and direct inhibition of sheep cardiac RyR channels by EPA, DHA and oleic acid.The Journal of membrane biology · 2003Article
- The random-coil 'C' fragment of the dihydropyridine receptor II-III loop can activate or inhibit native skeletal ryanodine receptors.The Biochemical journal · 2003Article
- Regulation of the calcium release channel from skeletal muscle by suramin and the disulfonated stilbene derivatives DIDS, DBDS, and DNDS.Biophysical journal · 2003Article
- The effects of HCl and CaCl(2) injections on intracellular calcium and pH in voltage-clamped snail (Helix aspersa) neurons.The Journal of general physiology · 2002Article
- Excitation-contraction coupling and fatigue mechanisms in skeletal muscle: studies with mechanically skinned fibres.Journal of muscle research and cell motility · 2002Review
- Regulation of the calcium release channel from rabbit skeletal muscle by the nucleotides ATP, AMP, IMP and adenosine.The Journal of physiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ryanodine receptor (RyR)-Ca(2+) release channels from rabbit skeletal muscle were incorporated into lipid bilayers. The effects of cytoplasmic and luminal pH were studied separately over the pH range 5-8, using half-unit intervals. RyR activity (at constant luminal pH of 7.5) was inhibited at acidic cytoplasmic pH, with a half-inhibitory pH (pH(I)) approximately 6.5, irrespective of bilayer potential and of whether the RyRs were activated by cytoplasmic Ca(2+) (50 microM), ATP (2 or 5 mM), or both. Inhibition occurred within approximately 1 s and could be fully reversed within approximately 1 s after brief inhibition or within approximately 30-60 s after longer exposure to acidic cytosolic pH. There was no evidence of any hysteresis in the cytoplasmic pH effect. Ryanodine-modified channels were less sensitive to pH inhibition, with pH(I) at approximately 5.5, but the inhibition was similarly reversible. Steady-state open and closed dwell times of RyRs during cytoplasmic pH inhibition suggest a mechanism where the binding of one proton inhibits the channel and the binding of two to three additional protons promotes further inhibited states. RyR activity was unaffected by luminal pH in the pH range 7.5 to 6.0. At lower luminal pH (5-5.5) most RyRs were completely inhibited, and raising the pH again produced partial to full recovery in only approximately 50% of cases, with the extent of recovery not detectably different between pH 7.5 and pH 9. The results indicate that isolated skeletal muscle RyRs are not inhibited as strongly by low cytoplasmic and luminal pH, as suggested by previous single-channel studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.