ArticleBiophysical journal2000
Mechanics of living cells measured by laser tracking microrheology.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 146 papers.
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Who cites it
146 citing papers in PubMed, 517 citations in OpenAlex.
- Optimal transitions between nonequilibrium steady states.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025Review
- A Simulation of the Mechanical Testing of the Cell Membrane and Cytoskeleton.Micromachines · 2024Article
- Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond.ACS photonics · 2024Review
- Studying fluctuating trajectories of optically confined passive tracers inside cells provides familiar active forces.Biomedical optics express · 2023Article
- Ultra-wideband optical coherence elastography from acoustic to ultrasonic frequencies.Nature communications · 2023Article
- Kappa-Carrageenan/Chitosan/Gelatin Scaffolds Provide a Biomimetic Microenvironment for Dentin-Pulp Regeneration.International journal of molecular sciences · 2023Article
- Review
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Review
- Article
- Multi-frequency passive and active microrheology with optical tweezers.Scientific reports · 2021Article
- Article
- Glass-like characteristics of intracellular motion in human cells.Biophysical journal · 2021Article
- Tumor cell nuclei soften during transendothelial migration.Journal of biomechanics · 2021Article
- Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport.Molecular biology of the cell · 2021Article
- Nonlinear Elastic and Inelastic Properties of Cells.Journal of biomechanical engineering · 2020Review
- Mapping cellular-scale internal mechanics in 3D tissues with thermally responsive hydrogel probes.Nature communications · 2020Article
86 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To establish laser-tracking microrheology (LTM) as a new technique for quantifying cytoskeletal mechanics, we measure viscoelastic moduli with wide bandwidth (5 decades) within living cells. With the first subcellular measurements of viscoelastic phase angles, LTM provides estimates of solid versus liquid behavior at different frequencies. In LTM, the viscoelastic shear moduli are inferred from the Brownian motion of particles embedded in the cytoskeletal network. Custom laser optoelectronics provide sub-nanometer and near-microsecond resolution of particle trajectories. The kidney epithelial cell line, COS7, has numerous spherical lipid-storage granules that are ideal probes for noninvasive LTM. Although most granules are percolating through perinuclear spaces, a subset of perinuclear granules is embedded in dense viscoelastic cytoplasm. Over all time scales embedded particles exhibit subdiffusive behavior and are not merely tethered by molecular motors. At low frequencies, lamellar regions (820 +/- 520 dyne/cm(2)) are more rigid than viscoelastic perinuclear regions (330 +/- 250 dyne/cm(2), p < 0.0001), but spectra converge at high frequencies. Although the actin-disrupting agent, latrunculin A, softens and liquefies lamellae, physiological levels of F-actin, alone (11 +/- 1.2 dyne/cm(2)) are approximately 70-fold softer than lamellae. Therefore, F-actin is necessary for lamellae mechanics, but not sufficient. Furthermore, in time-lapse of apparently quiescent cells, individual lamellar granules can show approximately 4-fold changes in moduli that last >10 s. Over a broad range of frequencies (0.1-30, 000 rad/s), LTM provides a unique ability to noninvasively quantify dynamic, local changes in cell viscoelasticity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.