ArticleBiophysical journal2000
Two-dimensional fluorescence intensity distribution analysis: theory and applications.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 153 citations in OpenAlex.
- Targeting NRF2 to treat cancer.Seminars in cancer biology · 2021Review
- Scanning single-molecule counting system for Eprobe with highly simple and effective approach.PloS one · 2020Article
- Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins.Biophysical journal · 2019Article
- Resolving fluorescent species by their brightness and diffusion using correlated photon-counting histograms.PloS one · 2019Article
- Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG.Biophysical journal · 2017Article
- Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.Molecular biology of the cell · 2017Article
- High Precision FRET at Single-molecule Level for Biomolecule Structure Determination.Journal of visualized experiments : JoVE · 2017Article
- Guanylate binding proteins directly attack Toxoplasma gondii via supramolecular complexes.eLife · 2016Article
- Quantitative localization microscopy: effects of photophysics and labeling stoichiometry.PloS one · 2015Article
- τFCS: multi-method global analysis enhances resolution and sensitivity in fluorescence fluctuation measurements.PloS one · 2014Article
- Time-averaged fluorescence intensity analysis in fluorescence fluctuation polarization sensitive experiments.Biomedical optics express · 2013Article
- The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing.The EMBO journal · 2013Article
- Simultaneous diffusion and brightness measurements and brightness profile visualization from single fluorescence fluctuation traces of GFP in living cells.European biophysics journal : EBJ · 2012Article
- Towards mimicking short linear peptide motifs: identification of new mixed α,β-peptidomimetic ligands for SLAM-Associated Protein (SAP) by confocal on-bead screening.Journal of chemical biology · 2012Article
- The spectroscopic basis of fluorescence triple correlation spectroscopy.The journal of physical chemistry. B · 2012Article
- Fluorescence correlation spectroscopy: past, present, future.Biophysical journal · 2011Review
- High-throughput screening of one-bead-one-compound libraries: identification of cyclic peptidyl inhibitors against calcineurin/NFAT interaction.ACS combinatorial science · 2011Article
- Lessons in fluctuation correlation spectroscopy.Annual review of physical chemistry · 2011Review
- Detection of siRNA administered to cells and animals by using a fluorescence intensity distribution analysis polarization system.Nucleic acids research · 2009Article
- Different species of alpha-synuclein oligomers induce calcium influx and seeding.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method of sample analysis is presented which is based on fitting a joint distribution of photon count numbers. In experiments, fluorescence from a microscopic volume containing a fluctuating number of molecules is monitored by two detectors, using a confocal microscope. The two detectors may have different polarizational or spectral responses. Concentrations of fluorescent species together with two specific brightness values per species are determined. The two-dimensional fluorescence intensity distribution analysis (2D-FIDA), if used with a polarization cube, is a tool that is able to distinguish fluorescent species with different specific polarization ratios. As an example of polarization studies by 2D-FIDA, binding of 5'-(6-carboxytetramethylrhodamine) (TAMRA)-labeled theophylline to an anti-theophylline antibody has been studied. Alternatively, if two-color equipment is used, 2D-FIDA can determine concentrations and specific brightness values of fluorescent species corresponding to individual labels alone and their complex. As an example of two-color 2D-FIDA, binding of TAMRA-labeled somatostatin-14 to the human type-2 high-affinity somatostatin receptors present in stained vesicles has been studied. The presented method is unusually accurate among fluorescence fluctuation methods. It is well suited for monitoring a variety of molecular interactions, including receptors and ligands or antibodies and antigens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.