ArticleMolecular and cellular biology2000
Functionally significant secondary structure of the simian virus 40 late polyadenylation signal.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Vector competence of temperate and tropical mosquito species for Babanki virus.PLoS neglected tropical diseases · 2026Article
- pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.ACS synthetic biology · 2024Article
- Rational design and construction of multi-copy biomanufacturing islands in mammalian cells.Nucleic acids research · 2022Article
- Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Regulation of closely juxtaposed proto-oncogeneRNA (New York, N.Y.) · 2021Article
- Sequence determinants of polyadenylation-mediated regulation.Genome research · 2019Article
- Conserved 3' UTR stem-loop structure in L1 and Alu transposons in human genome: possible role in retrotransposition.BMC genomics · 2016Article
- CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3' processing.Genes & development · 2014Article
- Article
- Bioinformatics in new generation flavivirus vaccines.Journal of biomedicine & biotechnology · 2010Article
- Distance-dependent processing of adeno-associated virus type 5 RNA is controlled by 5' exon definition.Journal of virology · 2007Article
- Article
- Multiple features contribute to the use of the immunoglobulin M secretion-specific poly(A) signal but are not required for developmental regulation.Molecular and cellular biology · 2006Article
- A downstream polyadenylation element in human papillomavirus type 16 L2 encodes multiple GGG motifs and interacts with hnRNP H.Journal of virology · 2005Article
- Secondary structure as a functional feature in the downstream region of mammalian polyadenylation signals.Molecular and cellular biology · 2004Article
- Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.The EMBO journal · 2004Article
- Transactivation of a viral target gene by herpes simplex virus ICP27 is posttranscriptional and does not require the endogenous promoter or polyadenylation site.Journal of virology · 2003Article
- Downstream elements of mammalian pre-mRNA polyadenylation signals: primary, secondary and higher-order structures.Nucleic acids research · 2003Review
- Characterization of specific protein-RNA complexes associated with the coupling of polyadenylation and last-intron removal.Molecular and cellular biology · 2002Article
- Means to an end: mechanisms of alternative polyadenylation of messenger RNA precursors.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
The structure of the highly efficient simian virus 40 late polyadenylation signal (LPA signal) is more complex than those of most known mammalian polyadenylation signals. It contains efficiency elements both upstream and downstream of the AAUAAA region, and the downstream region contains three defined elements (two U-rich elements and one G-rich element) instead of the single U- or GU-rich element found in most polyadenylation signals. Since many reports have indicated that the secondary structure in RNA may play a significant role in RNA processing, we have used nuclease structure analysis techniques to determine the secondary structure of the LPA signal. We find that the LPA signal has a functionally significant secondary structure. Much of the region upstream of AAUAAA is sensitive to single-strand-specific nucleases. The region downstream of AAUAAA has both double- and single-stranded characteristics. Both U-rich elements are predominately sensitive to the double-strand-specific nuclease RNase V(1), while the G-rich element is primarily single stranded. The U-rich element closest to AAUAAA contains four distinct RNase V(1)-sensitive regions, which we have designated structural region 1 (SR1), SR2, SR3, and SR4. Linker scanning mutants in the downstream region were analyzed both for structure and for function by in vitro cleavage analyses. These data show that the ability of the downstream region, particularly SR3, to form double-stranded structures correlates with efficient in vitro cleavage. We discuss the possibility that secondary structure downstream of the AAUAAA may be important for the functions of polyadenylation signals in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.