ArticleMolecular and cellular biology2000
Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Revisiting the question: When is a centromere not a kinetochore?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.Open biology · 2024Article
- Delineating the contribution of Spc105-bound PP1 to spindle checkpoint silencing and kinetochore microtubule attachment regulation.The Journal of cell biology · 2019Article
- Transposable Element Domestication As an Adaptation to Evolutionary Conflicts.Trends in genetics : TIG · 2017Review
- Review
- The fission yeast CENP-B protein Abp1 prevents pervasive transcription of repetitive DNA elements.Biochimica et biophysica acta · 2016Article
- The Long Terminal Repeat Retrotransposons Tf1 and Tf2 of Schizosaccharomyces pombe.Microbiology spectrum · 2015Review
- A regulatory effect of INMAP on centromere proteins: antisense INMAP induces CENP-B variation and centromeric halo.PloS one · 2014Article
- CENP-B cooperates with Set1 in bidirectional transcriptional silencing and genome organization of retrotransposons.Molecular and cellular biology · 2012Article
- Centromeres of filamentous fungi.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Putative CENP-B paralogues are not present at mammalian centromeres.Chromosoma · 2012Article
- CaMtw1, a member of the evolutionarily conserved Mis12 kinetochore protein family, is required for efficient inner kinetochore assembly in the pathogenic yeast Candida albicans.Molecular microbiology · 2011Article
- Article
- Article
- The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.The EMBO journal · 2008Article
- Convergent domestication of pogo-like transposases into centromere-binding proteins in fission yeast and mammals.Molecular biology and evolution · 2008Article
- The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.Cell division · 2006Article
- Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.Current genetics · 2006Article
- Inner kinetochore of the pathogenic yeast Candida glabrata.Eukaryotic cell · 2004Article
- Kinetochore and heterochromatin domains of the fission yeast centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
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2 authors at 1 institution in 1 country.
Funding
Abstract
Two functionally important DNA sequence elements in centromeres of the fission yeast Schizosaccharomyces pombe are the centromeric central core and the K-type repeat. Both of these DNA elements show internal functional redundancy that is not correlated with a conserved DNA sequence. Specific, but degenerate, sequences in these elements are bound in vitro by the S. pombe DNA-binding proteins Abp1p (also called Cbp1p) and Cbhp, which are related to the mammalian centromere DNA-binding protein CENP-B. In this study, we determined that Abp1p binds to at least one of its target sequences within S. pombe centromere II central core (cc2) DNA with an affinity (K(s) = 7 x 10(9) M(-1)) higher than those of other known centromere DNA-binding proteins for their cognate targets. In vivo, epitope-tagged Cbhp associated with centromeric K repeat chromatin, as well as with noncentromeric regions. Like abp1(+)/cbp1(+), we found that cbh(+) is not essential in fission yeast, but a strain carrying deletions of both genes (Deltaabp1 Deltacbh) is extremely compromised in growth rate and morphology and missegregates chromosomes at very high frequency. The synergism between the two null mutations suggests that these proteins perform redundant functions in S. pombe chromosome segregation. In vitro assays with cell extracts with these proteins depleted allowed the specific assignments of several binding sites for them within cc2 and the K-type repeat. Redundancy observed at the centromere DNA level appears to be reflected at the protein level, as no single member of the CENP-B-related protein family is essential for proper chromosome segregation in fission yeast. The relevance of these findings to mammalian centromeres is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.