ArticleMolecular and cellular biology2000
Recruitment of a basal polyadenylation factor by the upstream sequence element of the human lamin B2 polyadenylation signal.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- A conserved 3'UTR short motif regulates gene expression in vertebrates.Nucleic acids research · 2026Article
- Modulation of diverse biological processes by CPSF, the master regulator of mRNA 3' ends.RNA (New York, N.Y.) · 2024Review
- Cell Cycle Kinase Polo Is Controlled by a Widespread 3' Untranslated Region Regulatory Sequence in Drosophila melanogaster.Molecular and cellular biology · 2019Article
- Identification of A Gene Set Associated with Colorectal Cancer in Microarray Data Using The Entropy Method.Cell journal · 2019Article
- Useful Bicistronic Reporter System for Studying Poly(A) Site-Defining cis Elements and Regulation of Alternative Polyadenylation.International journal of molecular sciences · 2018Article
- Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex.eLife · 2017Article
- Article
- Processing and transcriptome expansion at the mRNA 3' end in health and disease: finding the right end.Pflugers Archiv : European journal of physiology · 2016Review
- Analysis of RNA processing reactions using cell free systems: 3' end cleavage of pre-mRNA substrates in vitro.Journal of visualized experiments : JoVE · 2014Article
- mRNA 3'end processing: A tale of the tail reaches the clinic.EMBO molecular medicine · 2014Review
- αCP Poly(C) binding proteins act as global regulators of alternative polyadenylation.Molecular and cellular biology · 2013Article
- Long-distance regulation of Add2 pre-mRNA3'end processing.RNA biology · 2013Article
- Novel upstream and downstream sequence elements contribute to polyadenylation efficiency.RNA biology · 2012Article
- Ending the message: poly(A) signals then and now.Genes & development · 2011Review
- An RNA-protein complex links enhanced nuclear 3' processing with cytoplasmic mRNA stabilization.The EMBO journal · 2011Article
- A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence.The EMBO journal · 2010Article
- Molecular mechanisms of eukaryotic pre-mRNA 3' end processing regulation.Nucleic acids research · 2010Review
- HIV-1 mRNA 3' end processing is distinctively regulated by eIF3f, CDK11, and splice factor 9G8.Molecular cell · 2009Article
- A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.Nucleic acids research · 2009Article
- 3' end mRNA processing: molecular mechanisms and implications for health and disease.The EMBO journal · 2008Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated how the upstream sequence element (USE) of the lamin B2 poly(A) signal mediates efficient 3'-end formation. In vitro analysis demonstrates that this USE increases both the efficiency of 3'-end cleavage and the processivity of poly(A) addition. Cross-linking using selectively labeled synthetic RNAs confirms that cleavage stimulation factor interacts with the sequences downstream of the cleavage site, while electrophoresis mobility shift assays demonstrate that the USE directly stabilizes the binding of the cleavage and polyadenylation specificity factor to the poly(A) signal. Thus in common with other poly(A) signals, the lamin B2 USE directly enhances the binding of basal poly(A) factors. In addition, a novel 55-kDa protein binds to the USE and the core poly(A) signal and appears to inhibit cleavage. The binding activity of this factor appears to change during the cell cycle, being greatest in S phase, when the lamin B2 gene is transcribed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.