ArticleThe British journal of ophthalmology2000
Responses of different cell lines from ocular tissues to elevated hydrostatic pressure.
Article in The British journal of ophthalmology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 69 citations in OpenAlex.
- Vasoactive intestinal peptide reduces ocular hypertension by regulating tight junction of trabecular meshwork through Rab13/PKA signalling complex.Annals of medicine · 2025Article
- A lab-on-a-chip model of glaucoma.Brain and behavior · 2020Article
- Retinal ganglion cell-conditioned medium and surrounding pressure alters gene expression and differentiation of rat retinal progenitor cells.Molecular medicine reports · 2018Article
- Article
- Life under pressure: The role of ocular cribriform cells in preventing glaucoma.Experimental eye research · 2016Review
- Experimentally Induced Mammalian Models of Glaucoma.BioMed research international · 2015Review
- Complement expression in the retina is not influenced by short-term pressure elevation.Molecular vision · 2014Article
- ATP-sensitive potassium (KATP) channel activation decreases intraocular pressure in the anterior chamber of the eye.Investigative ophthalmology & visual science · 2011Article
- Hydrostatic pressure-dependent changes in cyclic AMP signaling in optic nerve head astrocytes from Caucasian and African American donors.Molecular vision · 2009Article
- Increased isolevuglandin-modified proteins in glaucomatous astrocytes.Molecular vision · 2009Article
- TRPV1: contribution to retinal ganglion cell apoptosis and increased intracellular Ca2+ with exposure to hydrostatic pressure.Investigative ophthalmology & visual science · 2009Article
- Elevated pressure triggers a physiological release of ATP from the retina: Possible role for pannexin hemichannels.Neuroscience · 2008Article
- Article
- Effects of Cyclooxygenase Inhibitors on Apoptotic Neuroretinal Cells.Biomarker insights · 2008Article
- Degenerative and apoptotic events at retinal and optic nerve level after experimental induction of ocular hypertension.Molecular and cellular biochemistry · 2007Article
- Mechanosensitivity and the eye: cells coping with the pressure.The British journal of ophthalmology · 2006Review
- Increased production of tumor necrosis factor-alpha by glial cells exposed to simulated ischemia or elevated hydrostatic pressure induces apoptosis in cocultured retinal ganglion cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND/
aimsMechanical forces are thought to induce cellular responses through activation of signalling pathways. Cells within the intraocular environment are exposed to constant changes in the levels of intraocular pressure. In this study, an attempt was made to determine the acute effects of elevated hydrostatic pressure on different intraocular cells grown in culture.
methodsDifferent cell lines derived from ocular tissues including non-pigmented and pigmented ciliary epithelium, trabecular meshwork, retina, and lamina cribrosa were incubated in a pressurised chamber at 50 mm Hg in a culture incubator at 37 degrees C for up to 6 hours. Control cells were incubated at atmospheric pressure. The viability of the cells was examined using their intracellular esterase activity. The morphology and cytoskeleton of the cells were investigated using microscopy and phalloidin staining. Adenylyl cyclase activity was assessed by measuring the conversion of [(3)H]-cAMP from [(3)H]-ATP in response to elevated hydrostatic pressure for 1-6 hours. In addition, at the end of incubation period under elevated hydrostatic pressure the recovery of adenylyl cyclase activity to control levels was examined.
resultsCell viability did not change following exposure to elevated hydrostatic pressure for 6 hours. Cells subjected to elevated hydrostatic pressure demonstrated morphological differences characterised by a more rounded shape and a redistribution of actin stress fibres that was most prominent in lamina cribrosa astrocytes. A time dependent increase in basal adenylyl cyclase activity, and a decrease in maximum forskolin stimulated activity were observed in all cell lines following exposure to elevated hydrostatic pressure.
conclusionThese observations demonstrate that cell lines from different ocular tissues are sensitive to changes in external pressure in vitro. They exhibit morphological and cytoskeletal changes as well as significant alterations of intracellular adenylyl cyclase activity following exposure to acute and sustained levels of elevated hydrostatic pressure of up to 6 hours' duration.
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