ArticleJournal of virology2000
A murine leukemia virus (MuLV) long terminal repeat derived from rhesus macaques in the context of a lentivirus vector and MuLV gag sequence results in high-level gene expression in human T lymphocytes.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.Gene therapy · 2015Article
- Gefitinib targets ZAP-70-expressing chronic lymphocytic leukemia cells and inhibits B-cell receptor signaling.Cell death & disease · 2014Article
- Suicide gene approach using a dual-expression lentiviral vector to enhance the safety of ex vivo gene therapy for bone repair.Gene therapy · 2014Article
- SHP-1 phosphatase is a critical regulator in preventing natural killer cell self-killing.PloS one · 2012Article
- Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter.Human gene therapy · 2010Article
- Live cell monitoring of hiPSC generation and differentiation using differential expression of endogenous microRNAs.PloS one · 2010Article
- Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex.Nucleic acids research · 2010Article
- IL-9 induces CCL11 expression via STAT3 signalling in human airway smooth muscle cells.PloS one · 2010Article
- Platelets and viruses: an ambivalent relationship.Cellular and molecular life sciences : CMLS · 2010Review
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- Stable reduction of CCR5 by RNAi through hematopoietic stem cell transplant in non-human primates.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Mouse salivary glands and human beta-defensin-2 as a study model for antimicrobial gene therapy: technical considerations.International journal of antimicrobial agents · 2006Article
- Capacity of human beta-defensin expression in gene-transduced and cytokine-induced cells.Biochemical and biophysical research communications · 2006Article
- beta-Adrenoreceptors reactivate Kaposi's sarcoma-associated herpesvirus lytic replication via PKA-dependent control of viral RTA.Journal of virology · 2005Article
- Human immunodeficiency virus type 1 (HIV-1) Vpr enhances expression from unintegrated HIV-1 DNA.Journal of virology · 2003Article
- Antibody-directed targeting of retroviral vectors via cell surface antigens.Journal of virology · 2001Article
- Envelope gene of the human endogenous retrovirus HERV-W encodes a functional retrovirus envelope.Journal of virology · 2001Article
- Lentivirus vector-mediated hematopoietic stem cell gene transfer of common gamma-chain cytokine receptor in rhesus macaques.Journal of virology · 2001Article
- Clinical gene therapy in hematology: past and future.International journal of hematology · 2001Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We constructed human immunodeficiency virus type 1 (HIV-1) vectors that will allow higher levels of gene expression in T cells. Gene expression under the control of an internal cytomegalovirus (CMV) immediate-early promoter in a self-inactivating lentiviral vector (CSCG) is 4- to 15-fold lower in T-cell lines (SUPT1 and CEMX174) than in non-lymphoid-cell lines (HeLa and 293T). This is in contrast to a Moloney murine leukemia virus (MoMLV)-based retrovirus vector (SRalphaLEGFP). We therefore replaced the internal CMV promoter of CSCG with three different murine oncoretroviral long terminal repeat (LTR) promoters-murine sarcoma virus (MSV), MoMLV (MLV), and the LTR (termed Rh-MLV) that is derived from the ampho-mink cell focus-forming (AMP/MCF) retrovirus in the serum of one rhesus macaque monkey that developed T-cell lymphoma following autologous transplantation of enriched bone marrow stem cells transduced with a retrovirus vector preparation containing replication-competent viruses (E. F. Vanin, M. Kaloss, C. Broscius, and A. W. Nienhuis, J. Virol. 68:4241-4250, 1994). We found that the combination of Rh-MLV LTR and a partial gag sequence of MoMLV (Deltagag(871-1612)) in CS-Rh-MLV-E gave the highest level of enhanced green fluorescent protein (EGFP) gene expression compared with MLV, MSV LTR, phosphoglycerate kinase, and CMV promoters in T-cell lines, as well as activated primary T cells. Interestingly, there was a further two- to threefold increase in EGFP expression (thus, 10-fold-higher expression than with CMV) when the Rh-MLV promoter and Deltagag(871-1612) were used in a self-inactivating-vector setting that has a further deletion in the U3 region of the HIV-1 LTR. These hybrid vectors should prove useful in gene therapy applications for T cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.