ArticleJournal of virology2000
Identification of antigenic proteins encoded by human herpesvirus 8 and seroprevalence in the general population and among patients with and without Kaposi's sarcoma.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Is human herpesvirus 8 infection more common in men than in women? Systematic review and meta-analysis.International journal of cancer · 2016Pooled it
- A Case of Long-undiagnosed Classic Kaposi's Sarcoma with Extensive Skin Eruptions.JMA journal · 2025Article
- An Immunocompetent HIV-Negative Elderly Patient with Low-Grade Fever, Generalized Lymphadenopathy, Splenomegaly, and Acute Phase Response: Do Not Forget Castleman Disease.Case reports in infectious diseases · 2021Article
- Primary human herpesvirus 8-negative effusion-based lymphoma: a large B-cell lymphoma with favorable prognosis.Blood advances · 2020Article
- KSHV/HHV8-positive large B-cell lymphomas and associated diseases: a heterogeneous group of lymphoproliferative processes with significant clinicopathological overlap.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2020Review
- Kaposi sarcoma.Nature reviews. Disease primers · 2019Review
- Infectious Agents As Markers of Human Migration toward the Amazon Region of Brazil.Frontiers in microbiology · 2017Review
- Domestic cats seropositive for Felis catus gammaherpesvirus 1 are often qPCR negative.Virology · 2016Article
- Human herpesvirus 8 (HHV-8) detected by nested polymerase chain reaction (PCR) in HIV patients with or without Kaposi's sarcoma. An analytic cross-sectional study.Sao Paulo medical journal = Revista paulista de medicina · 2015Article
- Luciferase immunoprecipitation systems for measuring antibodies in autoimmune and infectious diseases.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Establishment of a CD4-positive cell line from an AIDS-related primary effusion lymphoma.International journal of hematology · 2013Article
- Development of whole-virus multiplex luminex-based serological assays for diagnosis of infections with kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 homologs in macaques.Clinical and vaccine immunology : CVI · 2013Article
- Searching for biomarkers: humoral response profiling with luciferase immunoprecipitation systems.Expert review of proteomics · 2011Review
- Pathology of Kaposi's Sarcoma-Associated Herpesvirus Infection.Frontiers in microbiology · 2011Article
- Antibody-profiling technologies for studying humoral responses to infectious agents.Expert review of vaccines · 2010Review
- Four-antigen mixture containing v-cyclin for serological screening of human herpesvirus 8 infection.Clinical and vaccine immunology : CVI · 2009Article
- Development of an immunofluorescence assay using recombinant proteins expressed in insect cells to screen and confirm presence of human herpesvirus 8-specific antibodies.Clinical and vaccine immunology : CVI · 2008Article
- Latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus interacts with origin recognition complexes at the LANA binding sequence within the terminal repeats.Journal of virology · 2006Article
- Human herpesvirus 8--a novel human pathogen.Virology journal · 2005Review
- Poly(ADP-ribose) polymerase 1 binds to Kaposi's sarcoma-associated herpesvirus (KSHV) terminal repeat sequence and modulates KSHV replication in latency.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To establish a sensitive and specific antibody assay, potent antigenic proteins encoded by human herpesvirus 8 (HHV8) were studied. Fifteen recombinant HHV8-encoded proteins were produced as glutathione S-transferase fusion proteins. The sera from AIDS-associated Kaposi's sarcoma (KS) patients reacted with four proteins encoded by open reading frames (ORFs) K8.1, 59, 65, and 73 in a Western blot assay. An enzyme-linked immunosorbent assay (ELISA) using these four proteins as antigens (mixed-antigen ELISA) revealed that all 26 sera derived from KS patients (24 with and 2 without human immunodeficiency virus infection) became positive for anti-HHV8 antibodies. The presence of HHV8 was demonstrated in 14 (1. 4%) of 1,004 sera from the Japanese general population and 10 (1.9%) of 527 sera from patients without HHV8-associated diseases. The presence of immunoglobulin G (IgG) and IgM antibodies against HHV8 examined further by the mixed-antigen ELISA and Western blotting revealed IgG antibody in all ELISA-positive sera, while IgM antibody against ORF K8.1 was absent. These data suggest that the ORF 73 and 65 proteins are potent antigens for a sensitive serological assay.
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