ArticleThe Journal of experimental medicine2000
The interleukin 7 receptor is required for T cell receptor gamma locus accessibility to the V(D)J recombinase.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.
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Who cites it
34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 103 citations in OpenAlex.
- Variants in the IL7RA gene confer susceptibility to multiple sclerosis in Caucasians: evidence based on 9734 cases and 10436 controls.Scientific reports · 2017Pooled it
- Polymorphism in the interleukin-7 receptor-alpha and outcome after allogeneic hematopoietic cell transplantation with matched unrelated donor.Scandinavian journal of immunology · 2013Pooled it
- The Whole Blood Transcriptomic Analysis in Sickle Cell Disease Reveals RUNX3 as a Potential Marker for Vaso-Occlusive Crises.International journal of molecular sciences · 2025Article
- IL-7 promotes the formation of DNA double strand breaks and DNA repair in murine pro-B cells.Frontiers in immunology · 2025Article
- Food for thought: Nutrient metabolism controlling early T cell development.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Advances in IL-7 Research on Tumour Therapy.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Harnessing the Power of IL-7 to Boost T Cell Immunity in Experimental and Clinical Immunotherapies.Immune network · 2024Review
- Interleukin-7 receptor signaling is crucial for enhancer-dependent TCRδ germline transcription mediated through STAT5 recruitment.Frontiers in immunology · 2022Article
- αβ/γδ T cell lineage outcome is regulated by intrathymic cell localization and environmental signals.Science advances · 2021Article
- From thymus to periphery: Molecular basis of effector γδ-T cell differentiation.Immunological reviews · 2020Review
- Regulation of γδ T Cell Effector Diversification in the Thymus.Frontiers in immunology · 2020Review
- The Common Cytokine Receptor γ Chain Family of Cytokines.Cold Spring Harbor perspectives in biology · 2018Review
- Functions of Vγ4 T Cells and Dendritic Epidermal T Cells on Skin Wound Healing.Frontiers in immunology · 2018Review
- Asynchronous lineage priming determines commitment to T cell and B cell lineages in fetal liver.Nature immunology · 2017Article
- IL-7 signaling imparts polyfunctionality and stemness potential to CD4(+) T cells.Oncoimmunology · 2016Article
- Forging T-Lymphocyte Identity: Intersecting Networks of Transcriptional Control.Advances in immunology · 2016Review
- Mechanism of Action of IL-7 and Its Potential Applications and Limitations in Cancer Immunotherapy.International journal of molecular sciences · 2015Review
- Mixing Signals: Molecular Turn Ons and Turn Offs for Innate γδ T-Cells.Frontiers in immunology · 2014Review
- The inhibitory receptor BTLA controls γδ T cell homeostasis and inflammatory responses.Immunity · 2013Article
- Osteoblast-specific overexpression of human interleukin-7 rescues the bone mass phenotype of interleukin-7-deficient female mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Defects in the interleukin (IL)-7 signal transduction pathway lead to severe immunodeficiency in humans and in mice. In IL-7 receptor-deficient (IL-7R-/-) mice, lymphoid precursors show a reduced survival rate and variable/diversity/joining region V(D)J recombination is variously affected in different loci, being arrested in the T cell receptor (TCR)-gamma locus, aberrant in the immunoglobulin heavy chain (IgH) locus, and delayed in the TCR-beta locus. Here, we analyze the recombination defect of the TCR-gamma locus. Using ligation-mediated polymerase chain reaction, we sought intermediates of the recombination process. In the absence of the IL-7 signal, no initiation of recombination of the TCR-gamma locus was observed, whereas recombination intermediates at the TCR-beta locus could be detected. Thus, the failure to rearrange the TCR-gamma locus is due to a failure to initiate cleavage rather than a failure to religate broken DNA ends. V(D)J recombination was previously thought to begin at the pro-T2 stage of T cell development after the arrest of IL-7R-/- thymocytes at the pro-T1 stage. However, here we show that both TCR-gamma and -beta recombination intermediates are readily detectable in normal T1 cells, but only TCR-beta intermediates were detected in IL-7R-/- T1 cells, supporting a mechanistic role for IL-7 in TCR-gamma locus rearrangement. Since reduced recombination activating gene (rag) expression has been reported in the absence of the IL-7 signal, we directly tested whether the TCR-gamma locus is accessible to cleavage by recombinant Rag proteins in vitro. We found a reduction in chromatin accessibility for Rag-mediated cleavage in IL-7R-/- thymocytes compared with wild-type. Thus, IL-7 controls recombination at the TCR-gamma locus by regulating locus accessibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.