ArticleThe Biochemical journal2000
Protein kinase C-beta contributes to NADPH oxidase activation in neutrophils.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 174 citations in OpenAlex.
- Article
- Computational Elucidation of Human β-Defensin-2 as a Dual Inhibitor of MMP-9 and PKC-βII for Diabetic Wound Management.ACS omega · 2025Article
- Membrane potential dynamics of C5a-stimulated neutrophil granulocytes.Pflugers Archiv : European journal of physiology · 2024Article
- NADPH Oxidase 3: Beyond the Inner Ear.Antioxidants (Basel, Switzerland) · 2024Review
- Hyaluronan and Reactive Oxygen Species Signaling-Novel Cues from the Matrix?Antioxidants (Basel, Switzerland) · 2023Review
- Review
- MARCKS Is an Essential Regulator of Reactive Oxygen Species Production in the Monocytic Cell Type.Antioxidants (Basel, Switzerland) · 2022Article
- Identification of novel protein kinase C-βII inhibitors: virtual screening, molecular docking and molecular dynamics simulation studies.Journal of molecular modeling · 2022Article
- Neutrophil Extracellular Traps Exacerbate Ischemic Brain Damage.Molecular neurobiology · 2022Review
- Neutrophil-Specific Knockdown of β2 Integrins Impairs Antifungal Effector Functions and Aggravates the Course of Invasive Pulmonal Aspergillosis.Frontiers in immunology · 2022Article
- Calcium Entry through TRPV1: A Potential Target for the Regulation of Proliferation and Apoptosis in Cancerous and Healthy Cells.International journal of molecular sciences · 2020Review
- Oxidative Stress as the Main Target in Diabetic Retinopathy Pathophysiology.Journal of diabetes research · 2019Review
- Specific PKC βII inhibitor: one stone two birds in the treatment of diabetic foot ulcers.Bioscience reports · 2018Article
- RAGE-TLR Crosstalk Sustains Chronic Inflammation in Neurodegeneration.Molecular neurobiology · 2018Review
- The Role of Hypoxia-Inducible Factor 1 in Mild Cognitive Impairment.Cellular and molecular neurobiology · 2017Review
- Redox regulation of the actin cytoskeleton and its role in the vascular system.Free radical biology & medicine · 2017Review
- Activated protein C inhibits neutrophil extracellular trap formationThe Journal of biological chemistry · 2017Article
- Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in anFrontiers in veterinary science · 2017Article
- Neutrophils to the ROScue: Mechanisms of NADPH Oxidase Activation and Bacterial Resistance.Frontiers in cellular and infection microbiology · 2017Review
- PKC-η-MARCKS Signaling Promotes Intracellular Survival of UnopsonizedFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analysed the involvement of the beta isotype of the protein kinase C (PKC) family in the activation of NADPH oxidase in primary neutrophils. Using immunofluorescence and cell fractionation, PKC-beta is shown to be recruited to the plasma membrane upon stimulation with phorbol ester and to the phagosomal membrane upon phagocytosis of IgG-coated particles (Fcgamma-receptor stimulus). The time course of recruitment is similar to that of NADPH oxidase activation by these stimuli. The PKC-beta specific inhibitor 379196 inhibits the response to PMA as well as to IgG-coated bacteria. Partial inhibition occurs between 10 and 100 nM of inhibitor, the concentration at which PKC-beta, but not other PKC isotypes, is targeted. Neutrophils isolated from a mouse that lacks PKC-beta also showed an inhibition of NADPH oxidase activation by PMA and IgG-coated particles. The level of inhibition is comparable to that achieved with 379196 in human neutrophils. Thus the PKC-beta isotype mediates activation of NADPH oxidase by PMA and by stimulation of Fcgamma receptors in neutrophils.
Indexed as
Identifiers
10727429PMC1220958W1992774063What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.