ArticleThe Biochemical journal2000
5-Hydroxytryptamine1A receptor/Gibetagamma stimulates mitogen-activated protein kinase via NAD(P)H oxidase and reactive oxygen species upstream of src in chinese hamster ovary fibroblasts.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Review
- Serotonergic Regulation in Alzheimer's Disease.International journal of molecular sciences · 2025Review
- Effects of Dietary L-TRP on Immunity, Antioxidant Capacity and Intestinal Microbiota of the Chinese Mitten Crab (Metabolites · 2022Article
- A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues.Nature protocols · 2022Review
- The β2-adrenergic receptor-ROS signaling axis: An overlooked component of β2AR function?Biochemical pharmacology · 2020Review
- Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining.Journal of the American Chemical Society · 2018Article
- Tryptophan hydroxylase 1 and 5-HTMolecular cancer · 2016Article
- Article
- Psychedelics and Immunomodulation: Novel Approaches and Therapeutic Opportunities.Frontiers in immunology · 2015Review
- Unique role of NADPH oxidase 5 in oxidative stress in human renal proximal tubule cells.Redox biology · 2014Article
- The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS.PloS one · 2013Article
- Orchestrating redox signaling networks through regulatory cysteine switches.ACS chemical biology · 2010Review
- Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression.The Journal of biological chemistry · 2008Article
- Fluorescent probes for nitric oxide and hydrogen peroxide in cell signaling.Current opinion in chemical biology · 2007Review
- An overlooked connection: serotonergic mediation of estrogen-related physiology and pathology.BMC women's health · 2005Article
- A review of the neuroprotective properties of the 5-HT1A receptor agonist repinotan HCl (BAYx3702) in ischemic stroke.CNS drug reviews · 2005Review
- Tandospirone activates neuroendocrine and ERK (MAP kinase) signaling pathways specifically through 5-HT1A receptor mechanisms in vivo.Naunyn-Schmiedeberg's archives of pharmacology · 2005Article
- Redox control of signal transduction, gene expression and cellular senescence.Neurochemical research · 2004Review
- Discrete generation of superoxide and hydrogen peroxide by T cell receptor stimulation: selective regulation of mitogen-activated protein kinase activation and fas ligand expression.The Journal of experimental medicine · 2002Article
- Receptor heterodimerization: essential mechanism for platelet-derived growth factor-induced epidermal growth factor receptor transactivation.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The hypothesis of this work is that the 'serotonin' or 5-hydroxytryptamine (5-HT)(1A) receptor, which activates the extracellular signal-regulated kinase (ERK) through a G(i)betagamma-mediated pathway, does so through the intermediate actions of reactive oxygen species (ROS). Five criteria were shown to support a key role for ROS in the activation of ERK by the 5-HT(1A) receptor. (1) Antioxidants inhibit activation of ERK by 5-HT. (2) Application of cysteine-reactive oxidant molecules activates ERK. (3) The 5-HT(1A) receptor alters cellular redox properties, and generates both superoxide and hydrogen peroxide. (4) A specific ROS-producing enzyme [NAD(P)H oxidase] is involved in the activation of ERK. (5) There is specificity both in the effects of various chemical oxidizers, and in the putative location of the ROS in the ERK activation pathway. We propose that NAD(P)H oxidase is located in the ERK activation pathway stimulated by the transfected 5-HT(1A) receptor in Chinese hamster ovary (CHO) cells downstream of G(i)betagamma subunits and upstream of or at the level of the non-receptor tyrosine kinase, Src. Moreover, these experiments provide confirmation that the transfected human 5-HT(1A) receptor induces the production of ROS (superoxide and hydrogen peroxide) in CHO cells, and support the possibility that an NAD(P)H oxidase-like enzyme might be involved in the 5-HT-mediated generation of both superoxide and hydrogen peroxide.
Indexed as
Identifiers
10727402PMC1220931W2055558954What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.