ArticleProceedings of the National Academy of Sciences of the United States of America2000
BRCA1 interaction with RNA polymerase II reveals a role for hRPB2 and hRPB10alpha in activated transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Molecular Evolution of the Archaeal DNA-Dependent RNA Polymerase: Cooperative Changes in Subunit Composition and Specific Domains of Small Subunits.International journal of molecular sciences · 2026Article
- BARD1 recognizes pre-rRNA for DNA damage repair and rRNA biogenesis.The Journal of biological chemistry · 2026Article
- The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination.Cell discovery · 2023Article
- The antitumorigenic roles of BRCA1-BARD1 in DNA repair and replication.Nature reviews. Molecular cell biology · 2020Review
- BRCA2 Regulates Transcription Elongation by RNA Polymerase II to Prevent R-Loop Accumulation.Cell reports · 2018Article
- Rpb3 promotes hepatocellular carcinoma through its N-terminus.Oncotarget · 2014Article
- BRCA1 and Oxidative Stress.Cancers · 2014Article
- Host Cell Reactivation and Transcriptional Activation of Carboplatin-Modified BRCA1.Breast cancer : basic and clinical research · 2014Article
- The emerging roles of GPRC5A in diseases.Oncoscience · 2014Review
- Predicting physical interactions between protein complexes.Molecular & cellular proteomics : MCP · 2013Article
- Analysis of induced pluripotent stem cells from a BRCA1 mutant family.Stem cell reports · 2013Article
- BRCA1 protein and nucleolin colocalize in breast carcinoma tissue and cancer cell lines.The American journal of pathology · 2010Article
- MYC in breast tumor progression.Expert review of anticancer therapy · 2008Review
- Multiple mechanisms contribute to inhibit transcription in response to DNA damage.The Journal of biological chemistry · 2008Article
- A mechanism for transcriptional repression dependent on the BRCA1 E3 ubiquitin ligase.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Subcellular localization of RPB5-mediating protein and its putative functional partner.Molecular and cellular biology · 2004Article
- Expression of an amino-terminal BRCA1 deletion mutant causes a dominant growth inhibition in MCF10A cells.Oncogene · 2004Article
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.Oncogene · 2004Article
- Roles of BRCA1 and BRCA2 in homologous recombination, DNA replication fidelity and the cellular response to ionizing radiation.Oncogene · 2003Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The functions of most of the 12 subunits of the RNA polymerase II (Pol II) enzyme are unknown. In this study, we demonstrate that two of the subunits, hRPB2 and hRPB10alpha, mediate the regulated stimulation of transcription. We find that the transcriptional coactivator BRCA1 interacts directly with the core Pol II complex in vitro. We tested whether single subunits from Pol II would compete with the intact Pol II complex to inhibit transcription stimulated by BRCA1. Excess purified Pol II subunits hRPB2 or hRPB10alpha blocked BRCA1- and VP16-dependent transcriptional activation in vitro with minimal effect on basal transcription. No other Pol II subunits tested inhibited activated transcription in these assays. Furthermore, hRPB10alpha, but not hRPB2, blocked Sp1-dependent activation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.