ArticleProceedings of the National Academy of Sciences of the United States of America2000
Saturation of, and competition for entry into, the apical secretory pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- Retinal gene therapy using epiretinal AAV-containing fibrin hydrogel implants.Science advances · 2025Article
- Porcine Single-Eye Retinal Pigment Epithelium Cell Culture for Barrier and Polarity Studies.Cells · 2025Article
- Early Choriocapillaris Loss in a Porcine Model of RPE Cell Debridement Precedes Pathology That Simulates Advanced Macular Degeneration.Investigative ophthalmology & visual science · 2024Article
- Human placental glucose transport in fetoplacental growth and metabolism.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- Human placental GLUT1 glucose transporter expression and the fetal insulin-like growth factor axis in pregnancies complicated by diabetes.Biochimica et biophysica acta. Molecular basis of disease · 2019Article
- Targeting mitochondrial dysfunction in CNS injury using Methylene Blue; still a magic bullet?Neurochemistry international · 2017Review
- Delayed Methylene Blue Improves Lesion Volume, Multi-Parametric Quantitative Magnetic Resonance Imaging Measurements, and Behavioral Outcome after Traumatic Brain Injury.Journal of neurotrauma · 2016Article
- Differential effects of Best disease causing missense mutations on bestrophin-1 trafficking.Human molecular genetics · 2013Article
- Analysis of polarized membrane traffic in hepatocytes and hepatic cell lines.Current protocols in cell biology · 2012Article
- Sublytic membrane-attack-complex (MAC) activation alters regulated rather than constitutive vascular endothelial growth factor (VEGF) secretion in retinal pigment epithelium monolayers.The Journal of biological chemistry · 2011Article
- Clathrin adaptor AP1B controls adenovirus infectivity of epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Transient TWEAK overexpression leads to a general salivary epithelial cell proliferation.Oral diseases · 2009Article
- Polarized traffic of LRP1 involves AP1B and SNX17 operating on Y-dependent sorting motifs in different pathways.Molecular biology of the cell · 2009Article
- Differential sorting of human parathyroid hormone after transduction of mouse and rat salivary glands.Human gene therapy · 2008Article
- Polarized secretion of leukemia inhibitory factor.BMC cell biology · 2008Article
- Secretory cargo composition affects polarized secretion in MDCK epithelial cells.Molecular and cellular biochemistry · 2008Article
- Efficient electroporation of DNA and protein into confluent and differentiated epithelial cells in culture.Traffic (Copenhagen, Denmark) · 2007Article
- Isoproterenol increases sorting of parotid gland cargo proteins to the basolateral pathway.American journal of physiology. Cell physiology · 2007Article
- GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells.The Journal of cell biology · 2006Article
- Effect of human vasoactive intestinal peptide gene transfer in a murine model of Sjogren's syndrome.Annals of the rheumatic diseases · 2006Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
To investigate mechanisms of apical sorting in the secretory pathway of epithelial cells, we expressed varying amounts of the 165 amino acid isoform of vascular endothelial growth factor (VEGF(165)) and transforming growth factor beta1 (TGF-beta1) via replication defective adenoviruses. Apical sorting of both proteins was efficient at low expression levels but saturated or was reversed at high expression levels. High expression levels of TGF-beta1 were effective at competing VEGF(165) out of the apical pathway; however, VEGF(165) did not compete out TGF-beta1. Tunicamycin inhibition experiments showed that the apical polarity of VEGF(165) was independent of N-glycosylation. We conclude that the apical sorting of these two molecules is a saturable, signal-mediated process, involving competition for apical sorting receptors. The sorting of the two proteins does not appear to involve N-glycans as sorting signals, or lectin sorters. The observations are particularly relevant to gene therapy because they demonstrate that overexpression of a transgene can result in undesirable missorting of the encoded protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.