ArticleProceedings of the National Academy of Sciences of the United States of America2000
The integral membrane S-locus receptor kinase of Brassica has serine/threonine kinase activity in a membranous environment and spontaneously forms oligomers in planta.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 112 citations in OpenAlex.
- A pollen selection system links self and interspecific incompatibility in the Brassicaceae.Nature ecology & evolution · 2024Article
- Stop and go signals at the stigma-pollen interface of the Brassicaceae.Plant physiology · 2023Article
- Paradigms of receptor kinase signaling in plants.The Biochemical journal · 2023Review
- Molecular plant immunity against biotrophic, hemibiotrophic, and necrotrophic fungi.Essays in biochemistry · 2022Review
- Amplification of cell signaling and disease resistance by an immunity receptor Ve1Ve2 heterocomplex in plants.Communications biology · 2022Article
- Characterization of a CommonPlants (Basel, Switzerland) · 2021Article
- Genome-wide identification and evolutionary analysis of RLKs involved in the response to aluminium stress in peanut.BMC plant biology · 2021Article
- Generation of Transgenic Self-IncompatiblePlants (Basel, Switzerland) · 2019Article
- Activation of the LRR Receptor-Like Kinase PSY1R Requires Transphosphorylation of Residues in the Activation Loop.Frontiers in plant science · 2017Article
- Structural basis for specific self-incompatibility response in Brassica.Cell research · 2016Article
- Yeast two-hybrid interactions between Arabidopsis lyrata S Receptor Kinase and the ARC1 E3 ligase.Plant signaling & behavior · 2016Article
- Salinity stress induces the production of 2-(2-phenylethyl)chromones and regulates novel classes of responsive genes involved in signal transduction in Aquilaria sinensis calli.BMC plant biology · 2016Article
- microRNA-dependent gene regulatory networks in maize leaf senescence.BMC plant biology · 2016Article
- Article
- Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility.Breeding science · 2014Review
- Genes associated with agronomic traits in non-heading Chinese cabbage identified by expression profiling.BMC plant biology · 2014Article
- A conserved role for the ARC1 E3 ligase in Brassicaceae self-incompatibility.Frontiers in plant science · 2014Review
- In planta assessment of the role of thioredoxin h proteins in the regulation of S-locus receptor kinase signaling in transgenic Arabidopsis.Plant physiology · 2013Article
- Thiol-based redox regulation in sexual plant reproduction: new insights and perspectives.Frontiers in plant science · 2013Review
- Progress towards elucidating the mechanisms of self-incompatibility in the grasses: further insights from studies in Lolium.Annals of botany · 2011Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To gain further insight into the mode of action of S-locus receptor kinase (SRK), a receptor-like kinase involved in the self-incompatibility response in Brassica, different recombinant SRK proteins have been expressed in a membranous environment using the insect cell/baculovirus system. Recombinant SRK proteins exhibited properties close to those of the endogenous stigmatic SRK protein and were found to autophosphorylate on serine and threonine residues in insect cell microsomes. Autophosphorylation was constitutive because it did not require the presence of pollen or stigma extracts in the phosphorylation buffer. Phosphorylation was shown to occur in trans, suggesting the existence of constitutive homooligomers of membrane-anchored recombinant SRK. To investigate the physiological relevance of these results, we have examined the oligomeric status of SRK in planta in cross-linking experiments and by velocity sedimentation on sucrose gradients. Our data strongly suggest that SRK is associated both with other SRK molecules and other stigma proteins in nonpollinated flowers. These findings may have important implications for our understanding of self-pollen signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.