ArticleProceedings of the National Academy of Sciences of the United States of America2000
RecA stimulates sister chromatid exchange and the fidelity of double-strand break repair, but not gene targeting, in plants transformed by Agrobacterium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 106 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- An update on precision genome editing by homology-directed repair in plants.Plant physiology · 2022Article
- Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Stimulation of homologous recombination in plants expressing heterologous recombinases.BMC plant biology · 2020Article
- Target-specific gene delivery in plant systems and their expression: Insights into recent developments.Journal of biosciences · 2020Review
- Precise gene replacement in plants through CRISPR/Cas genome editing technology: current status and future perspectives.aBIOTECH · 2020Review
- CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination.Plant cell reports · 2019Review
- Homology-Directed Repair of a DefectiveFrontiers in plant science · 2018Article
- A review on advanced methods in plant gene targeting.Journal, genetic engineering & biotechnology · 2017Review
- Homology-based double-strand break-induced genome engineering in plants.Plant cell reports · 2016Review
- Stable gene replacement in barley by targeted double-strand break induction.Journal of experimental botany · 2016Article
- Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants.Frontiers in plant science · 2016Article
- Precise Genome Modification via Sequence-Specific Nucleases-Mediated Gene Targeting for Crop Improvement.Frontiers in plant science · 2016Review
- DNA replicons for plant genome engineering.The Plant cell · 2014Article
- Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways.Genome research · 2013Article
- Coordinated phenotype switching with large-scale chromosome flip-flop inversion observed in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- In planta gene targeting.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Use of RecA fusion proteins to induce genomic modifications in zebrafish.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the bacterial RecA protein in plants stimulates homologous recombination in tobacco. Here we show that RecA plays a direct role in DNA strand exchange in vivo. The number of sister chromatid exchanges (SCEs) was increased 2.4-fold over wild type in transgenic tobacco plants expressing a nuclear-targeted RecA (nt-RecA) protein and could not be increased further by DNA damage, which caused a doubling of the baseline SCE frequency in wild-type plants. Although gene targeting requires homologous recombination, the number of targeted gene replacements was not increased markedly by the presence of nt-RecA by using Agrobacterium-mediated transformation. However, the number of double-strand breaks that were repaired at both sides by homologous recombination was increased 3.3-fold. Stimulation of SCE and fidelity of double-strand break repair by nt-RecA, but not by gene targeting, suggests that the stimulatory activity of RecA is linked to active DNA synthesis. Therefore, nascent replication-associated single strands may be a prerequisite for RecA action in plant cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.