ArticleProceedings of the National Academy of Sciences of the United States of America2000
Synthetic promoter elements obtained by nucleotide sequence variation and selection for activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Identification of functional cis-regulatory elements by sequential enrichment from a randomized synthetic DNA library.BMC plant biology · 2013Article
- A computational pipeline for identifying kinetic motifs to aid in the design and improvement of synthetic gene circuits.BMC bioinformatics · 2013Article
- Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay.Nature biotechnology · 2012Article
- Development and functional analysis of novel genetic promoters using DNA shuffling, hybridization and a combination thereof.PloS one · 2012Article
- Computational design of synthetic regulatory networks from a genetic library to characterize the designability of dynamical behaviors.Nucleic acids research · 2011Article
- Enhancement of reporter gene detection sensitivity by insertion of specific mini-peptide-coding sequences.Cancer gene therapy · 2010Article
- Up-regulation of activating transcription factor-5 suppresses SAP expression to activate T cells in hemophagocytic syndrome associated with Epstein-Barr virus infection and immune disorders.The American journal of pathology · 2008Article
- Construction and functional characteristics of tuber-specific and cold-inducible chimeric promoters in potato.Plant cell reports · 2008Article
- A positive feedback vector for identification of nucleotide sequences that enhance translation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The origin of subfunctions and modular gene regulation.Genetics · 2005Article
- Identification of synthetic endothelial cell-specific promoters by use of a high-throughput screen.Journal of virology · 2004Article
- Identification of promoter regions in the human genome by using a retroviral plasmid library-based functional reporter gene assay.Genome research · 2003Article
- Upregulation of Id2, an oncogenic helix-loop-helix protein, is mediated by the chimeric EWS/ets protein in Ewing sarcoma.Oncogene · 2003Article
- Efficient marking of neural stem cell-derived neurons with a modified murine embryonic stem cell virus, MESV2.Gene therapy · 2002Article
- Identification of two short internal ribosome entry sites selected from libraries of random oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Ets target genes: past, present and future.Oncogene · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic transcriptional regulation in different cells involves large numbers and arrangements of cis and trans elements. To survey the number of cis regulatory elements that are active in different contexts, we have devised a high-throughput selection procedure permitting synthesis of active cis motifs that enhance the activity of a minimal promoter. This synthetic promoter construction method (SPCM) was used to identify >100 DNA sequences that showed increased promoter activity in the neuroblastoma cell line Neuro2A. After determining DNA sequences of selected synthetic promoters, database searches for known elements revealed a predominance of eight motifs: AP2, CEBP, GRE, Ebox, ETS, CREB, AP1, and SP1/MAZ. The most active of the selected synthetic promoters contain composites of a number of these motifs. Assays of DNA binding and promoter activity of three exemplary motifs (ETS, CREB, and SP1/MAZ) were used to prove the effectiveness of SPCM in uncovering active sequences. Up to 10% of 133 selected active sequences had no match in currently available databases, raising the possibility that new motifs and transcriptional regulatory proteins to which they bind may be revealed by SPCM. The method may find uses in constructing databases of active cis motifs, in diagnostics, and in gene therapy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.